The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus

Naoaki Tsutsui, Yi Kyung Kim, Safiah Jasmani, Tsuyoshi Ohira, Marcy N. Wilder, Katsumi Aida

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

In order to compare the dynamics of vitellogenin gene expression between naturally maturing prawns and prawns induced to mature artificially by eyestalk ablation, a cDNA encoding vitellogenin was cloned from a cDNA library prepared from the hepatopancreas of the kuruma prawn Penaeus (Marsupenaeus) japonicus, and a quantitative real-time reverse transcription - polymerase chain reaction (RT-PCR) system was developed. Sequence analysis revealed the likely possibility that vitellogenin cDNA from the hepatopancreas was identical to that from the ovary which had been isolated in a previous study. Based on this information, a quantitative real-time RT-PCR system was established and the dynamics of vitellogenin mRNA levels were examined. In naturally maturing prawns, vitellogenin mRNA levels were maintained at low levels during the previtellogenic stage, and thereafter, levels increased as vitellogenesis progressed but decreased during the latter stages of maturation in the hepatopancreas and ovary. In contrast, in eyestalk-ablated prawns, changes in mRNA levels differed in both tissues; an obvious increment of mRNA levels was revealed in the ovary, whereas mRNA levels were negligible in the hepatopancreas. This suggests that eyestalk ablation cannot be used to accurately simulate the natural process of vitellogenin gene expression during vitellogenesis, and that vitellogenin gene expression is regulated in a tissue-specific manner.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalFisheries Science
Volume71
Issue number2
DOIs
Publication statusPublished - Apr 2005
Externally publishedYes

Fingerprint

Marsupenaeus japonicus
vitellogenin
gene expression
ablation
hepatopancreas
polymerase chain reaction
shrimp
vitellogenesis
maturation
quantitative polymerase chain reaction
reverse transcriptase polymerase chain reaction
cDNA libraries
sequence analysis
tissue

Keywords

  • Hepatopancreas
  • Ovary
  • Penaeus (Marsupenaeus) japonicus
  • Vitellogenin

ASJC Scopus subject areas

  • Aquatic Science

Cite this

The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus. / Tsutsui, Naoaki; Kim, Yi Kyung; Jasmani, Safiah; Ohira, Tsuyoshi; Wilder, Marcy N.; Aida, Katsumi.

In: Fisheries Science, Vol. 71, No. 2, 04.2005, p. 249-256.

Research output: Contribution to journalArticle

Tsutsui, Naoaki ; Kim, Yi Kyung ; Jasmani, Safiah ; Ohira, Tsuyoshi ; Wilder, Marcy N. ; Aida, Katsumi. / The dynamics of vitellogenin gene expression differs between intact and eyestalk ablated kuruma prawn Penaeus (Marsupenaeus) japonicus. In: Fisheries Science. 2005 ; Vol. 71, No. 2. pp. 249-256.
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