The dwarf phenotype of the Arabidopsis ac15 mutant is suppressed by a mutation in an upstream ORF of a bHLH gene

Akihiro Imai, Yoshie Hanzawa, Mio Komura, Kotaro T. Yamamoto, Yoshibumi Komeda, Taku Takahashi

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105 Citations (Scopus)

Abstract

Loss-of-function mutants of the Arabidopsis thaliana ACAULIS 5 (ACL5) gene, which encodes spermine synthase, exhibit a severe dwarf phenotype. To elucidate the ACL5-mediated regulatory pathways of stem internocle elongation, we isolated four suppressor of acaulis (sac) mutants that reverse the acl5 dwarf phenotype. Because these mutants do not rescue the dwarfism of known phytohormone-related mutants, the SAC genes appear to act specifically on the ACL5 pathways. We identify the gene responsible for the dominant sac51-d mutant, which almost completely suppresses the acl5 phenotype. sac51-d disrupts a short upstream open reading frame (uORF) of SAC51, which encodes a bHLH-type transcription factor. Our results indicate that premature termination of the uORF in sac51-d results in an increase in its own transcript level, probably as a result of an increased translation of the main ORF. We suggest a model in which ACL5 plays a role in the translational activation of SAC51, which may lead to the expression of a subset of genes required for stem elongation.

Original languageEnglish
Pages (from-to)3575-3585
Number of pages11
JournalDevelopment
Volume133
Issue number18
DOIs
Publication statusPublished - Sep 1 2006

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Keywords

  • Arabidopsis thaliana
  • Polyamine
  • Spermine
  • Stem elongation
  • Upstream ORF

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

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