The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain

Masaki Kuratani, Kanako Tanaka, Kazuhisa Terashima, Hajime Muraguchi, Takehito Nakazawa, Kiyoshi Nakahori, Takashi Kamada

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

The fruiting-body primordium of Coprinopsis cinerea exhibits remarkable photomorphogenesis. Under a 12-h light/12-h dark regime, the primordium proceeds to the fruiting-body maturation phase in which the primordium successively undergoes basidiospore formation, stipe elongation and pileus expansion, resulting in the mature fruiting-body. In continuous darkness, however, the primordium never proceeds to the maturation phase: the pileus and stipe tissues at the upper part of the primordium remain rudimentary while the basal part of the primordium elongates, producing the etiolated "dark stipe" phenotype. In our previous studies, blind mutants, which produce dark stipes under light conditions that promote fruiting-body maturation in the wild-type, have been isolated, and two genes, dst1 and dst2, responsible for the mutant phenotype have been identified. In this study we show that the dst2-1 mutant exhibits a blind phenotype during asexual spore production in addition to that in fruiting-body photomorphogenesis. We also reveal that dst2 is predicted to encode a protein with a putative flavin adenine dinucleotide (FAD)-binding-4 domain. The two blind phenotypes, together with the existence of an FAD-binding domain in Dst2, suggest that Dst2 may play a role in perceiving blue light.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalFungal Genetics and Biology
Volume47
Issue number2
DOIs
Publication statusPublished - Feb 2010

Fingerprint

Flavin-Adenine Dinucleotide
Essential Genes
Phenotype
Light
Proteins
Darkness
Spores
Genes

Keywords

  • Blue light
  • Coprinus cinereus
  • Dark stipe
  • Fruiting-body
  • Mushroom
  • Photoreceptor

ASJC Scopus subject areas

  • Genetics
  • Microbiology

Cite this

The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain. / Kuratani, Masaki; Tanaka, Kanako; Terashima, Kazuhisa; Muraguchi, Hajime; Nakazawa, Takehito; Nakahori, Kiyoshi; Kamada, Takashi.

In: Fungal Genetics and Biology, Vol. 47, No. 2, 02.2010, p. 152-158.

Research output: Contribution to journalArticle

Kuratani, Masaki ; Tanaka, Kanako ; Terashima, Kazuhisa ; Muraguchi, Hajime ; Nakazawa, Takehito ; Nakahori, Kiyoshi ; Kamada, Takashi. / The dst2 gene essential for photomorphogenesis of Coprinopsis cinerea encodes a protein with a putative FAD-binding-4 domain. In: Fungal Genetics and Biology. 2010 ; Vol. 47, No. 2. pp. 152-158.
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