The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco

Yusuke Kato, Shinya Murakami, Yumiko Yamamoto, Hiroshi Chatani, Yoshihiko Kondo, Takeshi Nakano, Akiho Yokota, Fumihiko Sato

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Plastids bear their own genome, organized into DNA-protein complexes (nucleoids). Recently, we identified a DNA-binding protease (CND41) in the chloroplast nucleoids of cultured tobacco (Nicotiana tabacum L.) cells. In this study, we examine the biochemical function of this novel DNA-binding protease, particularly in senescent leaves, because antisense tobacco with a reduced amount of CND41 showed retarded senescence. Nitrogen-depletion experiments clearly showed that CND41 antisense tobacco maintained green leaves and constant protein levels, especially ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), throughout the whole plant, whereas wildtype tobacco showed marked senescence and the reduction of protein levels in the lower leaves. In vitro analyses confirmed that CND41 showed proteolytic activity at physiological pH when denatured Rubisco was used as the substrate. These results suggest that CND41 is involved in Rubisco degradation and the translocation of nitrogen during senescence. The possible regulation of protease activity of CND41 through DNA-binding is discussed.

Original languageEnglish
Pages (from-to)97-104
Number of pages8
JournalPlanta
Volume220
Issue number1
DOIs
Publication statusPublished - Nov 2004
Externally publishedYes

Fingerprint

Oxygenases
ribulose 1,5-diphosphate
oxygenases
Tobacco
Peptide Hydrolases
tobacco
proteinases
degradation
DNA
leaves
Nitrogen
proteins
nitrogen
Proteins
Plastids
Nicotiana tabacum
plastids
Chloroplasts
chloroplasts
genome

Keywords

  • Aspartic protease
  • Chloroplast
  • Nicotiana
  • Nitrogen depletion
  • Rubisco degradation
  • Senescence

ASJC Scopus subject areas

  • Plant Science

Cite this

The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco. / Kato, Yusuke; Murakami, Shinya; Yamamoto, Yumiko; Chatani, Hiroshi; Kondo, Yoshihiko; Nakano, Takeshi; Yokota, Akiho; Sato, Fumihiko.

In: Planta, Vol. 220, No. 1, 11.2004, p. 97-104.

Research output: Contribution to journalArticle

Kato, Yusuke ; Murakami, Shinya ; Yamamoto, Yumiko ; Chatani, Hiroshi ; Kondo, Yoshihiko ; Nakano, Takeshi ; Yokota, Akiho ; Sato, Fumihiko. / The DNA-binding protease, CND41, and the degradation of ribulose-1,5-bisphosphate carboxylase/oxygenase in senescent leaves of tobacco. In: Planta. 2004 ; Vol. 220, No. 1. pp. 97-104.
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