The distributions of type IV collagen α chains in basement membranes of human epidermis and skin appendages

Haruko Hasegawa, Ichiro Naito, Kazuyo Nakano, Ryusuke Momota, Keiichiro Nishida, Takehito Taguchi, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka

Research output: Contribution to journalArticle

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Abstract

Distributions of type IV collagen α chains in the basement membrane (BM) of human skin and its appendages were analyzed by immunofluorescent microscopy using chain-specific monoclonal antibodies. The basement membrane beneath the epidermis contained [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) but no α3(IV) α4(IV) α5(IV); this held true for at the eccrine sweat glands and glandular ducts, sebaceous glands, hair follicles, and arrector muscles of hair. The secretary portion of the eccrine sweat glands was rich in [α1(IV)] 2 α2(IV) and had less [α5(IV)]2 α6(IV), while [α5(IV)]2 α6(IV) was abundant in the ductal portion. In the subepidermal zone, α5(IV)/ α6(IV) chain negative spots (1.9-15.0 μm) were frequently observed. Triple staining samples (Mel.2, α2(IV) and α5(IV) chains) showed that about 50% of epidermal melanocytes colocalized with such spots. Results suggest that these α5(IV)/ α6(IV) chain negative spots of the subepidermal basement membrane have a particular relationship with melanocytes and are sites for certain interactions between the two.

Original languageEnglish
Pages (from-to)255-265
Number of pages11
JournalArchives of Histology and Cytology
Volume70
Issue number4
DOIs
Publication statusPublished - 2007

Fingerprint

Collagen Type IV
Basement Membrane
Epidermis
Eccrine Glands
Sweat Glands
Melanocytes
Skin
Sebaceous Glands
Hair Follicle
Hair
Microscopy
Monoclonal Antibodies
Staining and Labeling
Muscles

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

The distributions of type IV collagen α chains in basement membranes of human epidermis and skin appendages. / Hasegawa, Haruko; Naito, Ichiro; Nakano, Kazuyo; Momota, Ryusuke; Nishida, Keiichiro; Taguchi, Takehito; Sado, Yoshikazu; Ninomiya, Yoshifumi; Ohtsuka, Aiji.

In: Archives of Histology and Cytology, Vol. 70, No. 4, 2007, p. 255-265.

Research output: Contribution to journalArticle

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