The distribution, organization and evolution of two abundant and widespread repetitive DNA sequences in the genus Hordeum

S. Taketa, H. Ando, K. Takeda, G. E. Harrison, J. S. Heslop-Harrison

    Research output: Contribution to journalArticlepeer-review

    58 Citations (Scopus)

    Abstract

    The genomic organization and chromosomal distributions of two abundant tandemly repeated DNA sequences, dpTa1 and pSc119.2, were examined in six wild Hordeum taxa, representing the four basic genomes of the genus, by Southern and fluorescence in situ hybridization. The dpTa1 probe hybridized to between 30 and 60 sites on the chromosomes of all five diploid species studied, but hybridization patterns differed among the species. Hybridization of the pSc119.2 sequence to the chromosomes and Southern blots of digested DNA detected signals in Hordeum bulbosum, Hordeum chilense, Hordeum marinum and Hordeum murinum 4x, but not in Hordeum murinum 2x and Hordeum vulgate ssp. spontaneum. A maximum of one pSc119.2 signal was observed in the terminal or subterminal region of each chromosome arm in the species carrying this sequence. The species carrying the same I-genome differed in the presence (Hordeum bulbosum) or absence (Hordeum spontaneum) of pSc119.2. The presence of pSc119.2 in the tetraploid cytotype of Hordeum murinum, but its absence in the diploid cytotype, suggests that the tetraploid is not likely to be a simple autotetraploid of the diploid. Data about the inter- and intra-specific variation of the two independent repetitive DNA sequences give information about both the interrelationships of the species and the evolution of the repetitive sequences.

    Original languageEnglish
    Pages (from-to)169-176
    Number of pages8
    JournalTheoretical and Applied Genetics
    Volume100
    Issue number2
    DOIs
    Publication statusPublished - Jan 1 2000

    Keywords

    • Barley
    • Hordeum
    • In situ hybridization
    • Phylogeny
    • Tandemly repeated DNA sequence

    ASJC Scopus subject areas

    • Biotechnology
    • Agronomy and Crop Science
    • Genetics

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