The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation

Taku Takahashi, A. Gasch, N. Nishizawa, N. H. Chua

Research output: Contribution to journalArticle

205 Citations (Scopus)

Abstract

We have isolated a recessive mutation named diminuto (dim) from T-DNA transformed lines of Arabidopsis thaliana. Under normal growth conditions, the dim mutant has very short hypocotyls, petioles, stems, and roots because of the reduced size of cells along the longitudinal axes of these organs. In addition, dim results in the development of open cotyledons and primary leaves in dark-grown seedlings. The gene for DIM was cloned by T-DNA tagging. DIM encodes a novel protein of 561 amino acids that possesses bipartite sequence domains characteristic of nuclear localization signals. Molecular and physiological studies indicate that the loss-of-function mutant allele does not abolish the response of seedlings to light or phytohormones, although the inhibitory effect of light on hypocotyl elongation is greater in the mutant than in wild type. Moreover, the dim mutation affects the expression of a β-tubulin gene, TUB1, which is thought to be important for plant cell growth. Our results suggest that the DIM gene product plays a critical role in the general process of plant cell elongation.

Original languageEnglish
Pages (from-to)97-107
Number of pages11
JournalGenes and Development
Volume9
Issue number1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Arabidopsis
Hypocotyl
Plant Cells
Seedlings
Genes
Light
Nuclear Localization Signals
Mutation
Plant Growth Regulators
Cotyledon
Tubulin
Growth
Cell Size
Alleles
Amino Acids
Proteins
T-DNA

Keywords

  • Arabidopsis mutant
  • cell elongation
  • nuclear localization signals
  • T-DNA tagging
  • tubuli genes

ASJC Scopus subject areas

  • Developmental Biology
  • Genetics

Cite this

The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. / Takahashi, Taku; Gasch, A.; Nishizawa, N.; Chua, N. H.

In: Genes and Development, Vol. 9, No. 1, 1995, p. 97-107.

Research output: Contribution to journalArticle

Takahashi, T, Gasch, A, Nishizawa, N & Chua, NH 1995, 'The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation', Genes and Development, vol. 9, no. 1, pp. 97-107.
Takahashi, Taku ; Gasch, A. ; Nishizawa, N. ; Chua, N. H. / The DIMINUTO gene of Arabidopsis is involved in regulating cell elongation. In: Genes and Development. 1995 ; Vol. 9, No. 1. pp. 97-107.
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