The differential distribution of type IV collagen α chains in the subepithelial basement membrane of the human alimentary canal

Hiroyuki Sato, Ichiro Naito, Ryusuke Momota, Yoshio Naomoto, Tomoki Yamatsuji, Yoshikazu Sado, Yoshifumi Ninomiya, Aiji Ohtsuka

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We studied distribution patterns of type IV collagen α chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using α(IV) chain-specific monoclonal antibodies. The α1(IV), α2(IV), α5(IV), and α6(IV) chains were found in the SBM throughout the tract, indicating the localization of [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) heterotrimeric molecules. The [α1(IV)]2 α2(IV) molecule was continuously stained, while the [α5(IV)]2 α6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of α3(IV) and α4(IV) chains which combined to form the α3(IV) α4(IV) α5(IV) heterotrimeric molecule with α5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an α3(IV) chain and α4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the α3(IV) α4(IV) α5(IV) molecule appears to function as a selective permeability barrier and /or as a protection against chemical stress from the luminal digestive enzymes.

Original languageEnglish
Pages (from-to)313-323
Number of pages11
JournalArchives of Histology and Cytology
Volume70
Issue number5
DOIs
Publication statusPublished - 2007

Fingerprint

Collagen Type IV
Basement Membrane
Epithelium
Large Intestine
Anal Canal
Gastric Mucosa
Esophagus
Small Intestine
Gastrointestinal Tract
Microscopy
Permeability
Stomach
Monoclonal Antibodies
Enzymes

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

The differential distribution of type IV collagen α chains in the subepithelial basement membrane of the human alimentary canal. / Sato, Hiroyuki; Naito, Ichiro; Momota, Ryusuke; Naomoto, Yoshio; Yamatsuji, Tomoki; Sado, Yoshikazu; Ninomiya, Yoshifumi; Ohtsuka, Aiji.

In: Archives of Histology and Cytology, Vol. 70, No. 5, 2007, p. 313-323.

Research output: Contribution to journalArticle

Sato, Hiroyuki ; Naito, Ichiro ; Momota, Ryusuke ; Naomoto, Yoshio ; Yamatsuji, Tomoki ; Sado, Yoshikazu ; Ninomiya, Yoshifumi ; Ohtsuka, Aiji. / The differential distribution of type IV collagen α chains in the subepithelial basement membrane of the human alimentary canal. In: Archives of Histology and Cytology. 2007 ; Vol. 70, No. 5. pp. 313-323.
@article{8801230cbc124321a3d0a73653451679,
title = "The differential distribution of type IV collagen α chains in the subepithelial basement membrane of the human alimentary canal",
abstract = "We studied distribution patterns of type IV collagen α chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using α(IV) chain-specific monoclonal antibodies. The α1(IV), α2(IV), α5(IV), and α6(IV) chains were found in the SBM throughout the tract, indicating the localization of [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) heterotrimeric molecules. The [α1(IV)]2 α2(IV) molecule was continuously stained, while the [α5(IV)]2 α6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of α3(IV) and α4(IV) chains which combined to form the α3(IV) α4(IV) α5(IV) heterotrimeric molecule with α5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an α3(IV) chain and α4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the α3(IV) α4(IV) α5(IV) molecule appears to function as a selective permeability barrier and /or as a protection against chemical stress from the luminal digestive enzymes.",
author = "Hiroyuki Sato and Ichiro Naito and Ryusuke Momota and Yoshio Naomoto and Tomoki Yamatsuji and Yoshikazu Sado and Yoshifumi Ninomiya and Aiji Ohtsuka",
year = "2007",
doi = "10.1679/aohc.70.313",
language = "English",
volume = "70",
pages = "313--323",
journal = "Archives of Histology and Cytology",
issn = "0914-9465",
publisher = "Japan Society of Histological Documentation",
number = "5",

}

TY - JOUR

T1 - The differential distribution of type IV collagen α chains in the subepithelial basement membrane of the human alimentary canal

AU - Sato, Hiroyuki

AU - Naito, Ichiro

AU - Momota, Ryusuke

AU - Naomoto, Yoshio

AU - Yamatsuji, Tomoki

AU - Sado, Yoshikazu

AU - Ninomiya, Yoshifumi

AU - Ohtsuka, Aiji

PY - 2007

Y1 - 2007

N2 - We studied distribution patterns of type IV collagen α chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using α(IV) chain-specific monoclonal antibodies. The α1(IV), α2(IV), α5(IV), and α6(IV) chains were found in the SBM throughout the tract, indicating the localization of [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) heterotrimeric molecules. The [α1(IV)]2 α2(IV) molecule was continuously stained, while the [α5(IV)]2 α6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of α3(IV) and α4(IV) chains which combined to form the α3(IV) α4(IV) α5(IV) heterotrimeric molecule with α5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an α3(IV) chain and α4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the α3(IV) α4(IV) α5(IV) molecule appears to function as a selective permeability barrier and /or as a protection against chemical stress from the luminal digestive enzymes.

AB - We studied distribution patterns of type IV collagen α chains in the subepithelial basement membrane (SBM) of the human gastrointestinal tract - the esophagus through the anal canal - by immunofluorescent microscopy using α(IV) chain-specific monoclonal antibodies. The α1(IV), α2(IV), α5(IV), and α6(IV) chains were found in the SBM throughout the tract, indicating the localization of [α1(IV)]2 α2(IV) and [α5(IV)]2 α6(IV) heterotrimeric molecules. The [α1(IV)]2 α2(IV) molecule was continuously stained, while the [α5(IV)]2 α6(IV) molecule was weakly stained in gastric glands and small intestinal crypts. In addition, the SBM at the luminal surface epithelium of the stomach and large intestine contained small amounts of α3(IV) and α4(IV) chains which combined to form the α3(IV) α4(IV) α5(IV) heterotrimeric molecule with α5(IV) chain. The SBM beneath the villous epithelium of the small intestine was also demonstrated to have an α3(IV) chain and α4(IV) chain. Considering the specific locations of the type IV collagen trimers throughout the gastrointestinal SBM, the supramolecular network containing the α3(IV) α4(IV) α5(IV) molecule appears to function as a selective permeability barrier and /or as a protection against chemical stress from the luminal digestive enzymes.

UR - http://www.scopus.com/inward/record.url?scp=42649127394&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42649127394&partnerID=8YFLogxK

U2 - 10.1679/aohc.70.313

DO - 10.1679/aohc.70.313

M3 - Article

C2 - 18431032

AN - SCOPUS:42649127394

VL - 70

SP - 313

EP - 323

JO - Archives of Histology and Cytology

JF - Archives of Histology and Cytology

SN - 0914-9465

IS - 5

ER -