The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis

Toshiko Ueda, Tsuyoshi Maekawa, Daikai Sadamitsu, Shuzo Oshita, Keiki Ogino, Kazuyuki Nakamura

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Abstract

The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis (CZE) is described. Electrophoresis was carried out in a 65 cm long, 75 μm wide silica capillary, using 750 mM sodium chloride containing 5% NICE‐Pak OFM Anion‐BT (an additive reversing the electroosmotic flow) as running buffer, at a potential of 20 kV, with on‐column UV detection at 214 nm. Under these conditions, the two anions migrated in the order of nitrite and nitrate, completely resolved from each other. Forty‐one samples of ultrafiltered blood plasma of healthy volunteers were analyzed. The concentrations of nitrite and nitrate were 0.15 ± 0.07 mg/L and 3.2 ± 1.6 mg/L (mean± SD), respectively, with a linear correlation in the range of 0.1–50 mg/L. The within‐day and between‐day coefficients of variation (CVs) for nitrite and nitrate were lower than 5% and 8%, respectively. The recovery was 97‐114%. The estimated plasma volume needed for each determination was 108 nL.

Original languageEnglish
Pages (from-to)1002-1004
Number of pages3
JournalELECTROPHORESIS
Volume16
Issue number1
DOIs
Publication statusPublished - 1995

Keywords

  • Capillary electrophoresis
  • Critical care
  • Nitric oxide
  • Plasma nitrate
  • Plasma nitrite

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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    Ueda, T., Maekawa, T., Sadamitsu, D., Oshita, S., Ogino, K., & Nakamura, K. (1995). The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis. ELECTROPHORESIS, 16(1), 1002-1004. https://doi.org/10.1002/elps.11501601167