The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis

T. Ueda, T. Maekawa, D. Sadamitsu, S. Oshita, Keiki Ogino, K. Nakamura

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Abstract

The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis (CZE) is described. Electrophoresis was carried out in a 65 cm long, 75 μm wide silica capillary, using 750 mM sodium chloride containing 5% NICE-Pak OFM Anion-BT (an additive reversing the electroosmotic flow) as running buffer, at a potential of 20 kV, with on- column UV detection at 214 nm. Under these conditions, the two anions migrated in the order of nitrite and nitrate, completely resolved from each other. Forty-one samples of ultrafiltered blood plasma of healthy volunteers were analyzed. The concentrations of nitrite and nitrate were 0.15 ± 0.07 mg/L and 3.2 ± 1.6 mg/L (mean ± SD), respectively, with a linear correlation in the range of 0.1-50 mg/L. The within-day and between-day coefficients of variation (CVs) for nitrite and nitrate were lower than 5% and 8%, respectively. The recovery was 97-114%. The estimated plasma volume needed for each determination was 108 nL.

Original languageEnglish
Pages (from-to)1002-1004
Number of pages3
JournalElectrophoresis
Volume16
Issue number6
Publication statusPublished - 1995
Externally publishedYes

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Keywords

  • Capillary electrophoresis
  • Critical care
  • Nitric oxide
  • Plasma nitrate
  • Plasma nitrite

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Ueda, T., Maekawa, T., Sadamitsu, D., Oshita, S., Ogino, K., & Nakamura, K. (1995). The determination of nitrite and nitrate in human blood plasma by capillary zone electrophoresis. Electrophoresis, 16(6), 1002-1004.