The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development

J. Q. Feng, H. Huang, Y. Lu, L. Ye, Y. Xie, T. W. Tsutsui, T. Kunieda, T. Castranio, G. Scott, L. B. Bonewald, Y. Mishina

Research output: Contribution to journalArticle

171 Citations (Scopus)

Abstract

Dentin Matrix Protein 1 (Dmp1) was originally identified from dentin. However, its expression and function in vivo are not clear. To clarify these two issues, we have generated mice carrying a truncated Dmp1 gene by using gene targeting to replace exon 6 with a lacZ gene. Northern blot analysis shows the expected 5.8-kb Dmp1-lacZ fusion transcript and loss of the wild-type 2.8-kb Dmp1 transcript, confirmed by a lack of immunostaining for the protein. Using heterozygous animals, we demonstrate that Dmp1 is specific for mineralized tissues. Not previously shown, Dmp1 is also expressed in pulp cells. Dmp1-deficient embryos and newborns display no apparent gross abnormal phenotype, although there are a modest expansion of the hypertrophic chondrocyte zone and a modest increase in the long bone diameter. This suggests that DMP1 is not essential for early mouse skeletal or dental development.

Original languageEnglish
Pages (from-to)776-780
Number of pages5
JournalJournal of dental research
Volume82
Issue number10
DOIs
Publication statusPublished - Oct 2003

Keywords

  • Bone
  • Development
  • Dmp1 expression
  • Knockout mice
  • Tooth

ASJC Scopus subject areas

  • Dentistry(all)

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    Feng, J. Q., Huang, H., Lu, Y., Ye, L., Xie, Y., Tsutsui, T. W., Kunieda, T., Castranio, T., Scott, G., Bonewald, L. B., & Mishina, Y. (2003). The dentin matrix protein 1 (Dmp1) is specifically expressed in mineralized, but not soft, tissues during development. Journal of dental research, 82(10), 776-780. https://doi.org/10.1177/154405910308201003