Abstract
Vibrio vulnificus can be divided into two groups on the basis of pathogenesis. Group 1 is pathogenic only to humans, whereas group 2 is pathogenic to eels and occasionally to humans. Although both groups produce a 50-kDa cytotoxin-hemolysin (V. vulnificus hemolysin; VVH), the toxins are different. In the present study, the nucleotide sequence of the toxin gene (vvhA) of strain CDC B3547 (a group 2 strain) was determined, and the deduced amino acid sequence was compared to that of strain L-180 (a group 1 strain). The nucleotide sequence of vvhA of strain CDC B3547 was about 96% identical with that of strain L-180, which results in a difference of 3 amino acid residues in the C-terminal lectin domain of VVH. Nevertheless, two primer sets for polymerase chain reaction could be designed to differentiate the toxin gene of each strain. When 27 V. vulnificus clinical isolates were tested, group 1 strains (9 strains) were shown to react only to the primers designed for vvhA of strain L-180; whereas, the gene of group 2 strains (18 strains) could be amplified with the primers for vvhA of strain CDC B3547. These findings may lead to development of a novel genetic grouping system related to the virulence potential or to the host range.
| Original language | English |
|---|---|
| Pages (from-to) | 513-519 |
| Number of pages | 7 |
| Journal | Microbiology and Immunology |
| Volume | 49 |
| Issue number | 6 |
| Publication status | Published - 2005 |
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Keywords
- Cytotoxin
- Genetic grouping
- Hemolysin
- Vibrio vulnificus
ASJC Scopus subject areas
- Immunology and Microbiology(all)
- Microbiology (medical)
- Microbiology
Cite this
The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains : Comparison of nucleotide sequences and application to the genetic grouping. / Senoh, Mitsutoshi; Miyoshi, Shin-ichi; Okamoto, Keinosuke; Fouz, Belen; Amaro, Carmen; Shinoda, Sumio.
In: Microbiology and Immunology, Vol. 49, No. 6, 2005, p. 513-519.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The cytotoxin-hemolysin genes of human and eel pathogenic Vibrio vulnificus strains
T2 - Comparison of nucleotide sequences and application to the genetic grouping
AU - Senoh, Mitsutoshi
AU - Miyoshi, Shin-ichi
AU - Okamoto, Keinosuke
AU - Fouz, Belen
AU - Amaro, Carmen
AU - Shinoda, Sumio
PY - 2005
Y1 - 2005
N2 - Vibrio vulnificus can be divided into two groups on the basis of pathogenesis. Group 1 is pathogenic only to humans, whereas group 2 is pathogenic to eels and occasionally to humans. Although both groups produce a 50-kDa cytotoxin-hemolysin (V. vulnificus hemolysin; VVH), the toxins are different. In the present study, the nucleotide sequence of the toxin gene (vvhA) of strain CDC B3547 (a group 2 strain) was determined, and the deduced amino acid sequence was compared to that of strain L-180 (a group 1 strain). The nucleotide sequence of vvhA of strain CDC B3547 was about 96% identical with that of strain L-180, which results in a difference of 3 amino acid residues in the C-terminal lectin domain of VVH. Nevertheless, two primer sets for polymerase chain reaction could be designed to differentiate the toxin gene of each strain. When 27 V. vulnificus clinical isolates were tested, group 1 strains (9 strains) were shown to react only to the primers designed for vvhA of strain L-180; whereas, the gene of group 2 strains (18 strains) could be amplified with the primers for vvhA of strain CDC B3547. These findings may lead to development of a novel genetic grouping system related to the virulence potential or to the host range.
AB - Vibrio vulnificus can be divided into two groups on the basis of pathogenesis. Group 1 is pathogenic only to humans, whereas group 2 is pathogenic to eels and occasionally to humans. Although both groups produce a 50-kDa cytotoxin-hemolysin (V. vulnificus hemolysin; VVH), the toxins are different. In the present study, the nucleotide sequence of the toxin gene (vvhA) of strain CDC B3547 (a group 2 strain) was determined, and the deduced amino acid sequence was compared to that of strain L-180 (a group 1 strain). The nucleotide sequence of vvhA of strain CDC B3547 was about 96% identical with that of strain L-180, which results in a difference of 3 amino acid residues in the C-terminal lectin domain of VVH. Nevertheless, two primer sets for polymerase chain reaction could be designed to differentiate the toxin gene of each strain. When 27 V. vulnificus clinical isolates were tested, group 1 strains (9 strains) were shown to react only to the primers designed for vvhA of strain L-180; whereas, the gene of group 2 strains (18 strains) could be amplified with the primers for vvhA of strain CDC B3547. These findings may lead to development of a novel genetic grouping system related to the virulence potential or to the host range.
KW - Cytotoxin
KW - Genetic grouping
KW - Hemolysin
KW - Vibrio vulnificus
UR - http://www.scopus.com/inward/record.url?scp=21744453937&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21744453937&partnerID=8YFLogxK
M3 - Article
C2 - 15965298
AN - SCOPUS:21744453937
VL - 49
SP - 513
EP - 519
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 6
ER -