The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor

Liying Sun, Andika Ida Bagus, Jiangfeng Shen, Di Yang, Claudio Ratti, Jianping Chen

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Some viruses use alternative translation initiation at non-AUG codons as a strategy to produce multiple proteins during gene expression. Here we show that, using this strategy, Chinese wheat mosaic virus (CWMV; Furovirus) expresses a larger form of coat protein (N-ext/CP) in infected plants. Site-directed mutagenesis and transient expression analysis confirmed that CWMV N-ext/CP is initiated at an upstream in-frame CUG codon at nucleotide position 207-209 of RNA 2, which adds a 39 amino acid (aa) N-terminal extension to the major CP. Interestingly, in planta and in vitro analyses indicated that CWMV N-ext/CP but not CP interacts with the CWMV cysteine-rich protein (CRP), an RNA silencing suppressor. We further determined that the N-terminal 39 aa extension, particularly the 10 aa region immediately upstream of the major CP coding region is responsible for the interaction of N-ext/CP with CRP. In an Agrobacterium co-infiltration assay, co-expression with N-ext/CP did not affect CRP silencing suppression activity. Thus the alternative translation initiation at a CUG codon provides the CWMV N-ext/CP with the ability to bind to the viral silencing suppressor.

Original languageEnglish
Pages (from-to)66-74
Number of pages9
JournalVirus Research
Volume177
Issue number1
DOIs
Publication statusPublished - Oct 2013
Externally publishedYes

Fingerprint

Mosaic Viruses
Capsid Proteins
RNA Interference
Triticum
Cysteine
Codon
Amino Acids
Proteins
Agrobacterium
Site-Directed Mutagenesis
Nucleotides
RNA
Viruses
Gene Expression

Keywords

  • Alternative translation initiation
  • Chinese wheat mosaic virus
  • CUG codon
  • Cysteine-rich suppressor
  • Furovirus
  • Larger form coat protein

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases
  • Cancer Research

Cite this

The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor. / Sun, Liying; Ida Bagus, Andika; Shen, Jiangfeng; Yang, Di; Ratti, Claudio; Chen, Jianping.

In: Virus Research, Vol. 177, No. 1, 10.2013, p. 66-74.

Research output: Contribution to journalArticle

Sun, Liying ; Ida Bagus, Andika ; Shen, Jiangfeng ; Yang, Di ; Ratti, Claudio ; Chen, Jianping. / The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor. In: Virus Research. 2013 ; Vol. 177, No. 1. pp. 66-74.
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