The crystal structure of l-lactate oxidase from Aerococcus viridans at 2.1 Å resolution reveals the mechanism of strict substrate recognition

Yasufumi Umena, Kazuko Yorita, Takeshi Matsuoka, Akiko Kita, Kiyoshi Fukui, Yukio Morimoto

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

l-Lactate oxidase (LOX) from Aerococcus viridans is a member of the α-hydroxyacid-oxidase flavoenzyme family. We have determined the three-dimensional structure of LOX and revealed the mechanism of substrate recognition. The LOX monomer structure has a typical α88 motif commonly found in other flavin family proteins. A related enzyme, glycolate oxidase, catalyzes the oxidation of glycolate rather than lactate. Comparison of the two enzyme structures highlights the importance of five residues around the FMN prosthetic group of LOX, which act synergistically to discriminate between the l/d configurations of lactate. X-ray crystallography of LOX gave a space group I422 of unit-cell parameters a = b = 191.096 Å, c = 194.497 Å and α = β = γ = 90° with four monomers per asymmetric unit. The four independent monomers display slight structural differences around the active site. Diffraction data were collected, under cryogenic conditions to 2.1 Å resolution at the synchrotron facilities in Japan.

Original languageEnglish
Pages (from-to)249-256
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume350
Issue number2
DOIs
Publication statusPublished - Nov 17 2006
Externally publishedYes

Fingerprint

lactate 2-monooxygenase
Aerococcus
Crystal structure
Substrates
glycolic acid
Monomers
Lactic Acid
Flavin Mononucleotide
Synchrotrons
X ray crystallography
X Ray Crystallography
Enzymes
Prosthetics
Cryogenics
Catalytic Domain
Japan
Oxidoreductases
Diffraction
Oxidation

Keywords

  • Flavoprotein
  • Lactate oxidase
  • Substrate recognition
  • X-ray structure analysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The crystal structure of l-lactate oxidase from Aerococcus viridans at 2.1 Å resolution reveals the mechanism of strict substrate recognition. / Umena, Yasufumi; Yorita, Kazuko; Matsuoka, Takeshi; Kita, Akiko; Fukui, Kiyoshi; Morimoto, Yukio.

In: Biochemical and Biophysical Research Communications, Vol. 350, No. 2, 17.11.2006, p. 249-256.

Research output: Contribution to journalArticle

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