The crystal structure of Deg9 reveals a novel octameric-type HtrA protease

Min Ouyang, Xiaoyi Li, Shun Zhao, Hua Pu, Jian-Ren Shen, Zach Adam, Tim Clausen, Lixin Zhang

Research output: Contribution to journalArticle

Abstract

The high temperature requirement A (HtrA) proteases (also termed Deg proteases) play important roles in diverse organisms by regulating protein quality and quantity. One of the 16 Arabidopsis homologs, Deg9, is located in the nucleus where it modulates cytokinin- A nd light-mediated signalling via degrading the ARABIDOPSIS RESPONSE REGULATOR 4 (ARR4). To uncover the structural features underlying the proteolytic activity of Deg9, we determined its crystal structure. Unlike the well-established trimeric building block of HtrAs, Deg9 displays a novel octameric structure consisting of two tetrameric rings that have distinct conformations. Based on the structural architecture, we generated several mutant variants of Deg9, determined their structure and tested their proteolytic activity towards ARR4. The results of the structural and biochemical analyses allowed us to propose a model for a novel mechanism of substrate recognition and activity regulation of Deg9. In this model, protease activation of one tetramer is mediated by en-bloc reorientation of the protease domains to open an entrance for the substrate in the opposite (inactive) tetramer. This study provides the structural basis for understanding how the levels of nuclear signal components are regulated by a plant protease.

Original languageEnglish
Pages (from-to)973-982
Number of pages10
JournalNature Plants
Volume3
Issue number12
DOIs
Publication statusPublished - Dec 1 2017

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crystal structure
proteinases
Arabidopsis
temperature
cytokinins
mutants
organisms
proteins

ASJC Scopus subject areas

  • Plant Science

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The crystal structure of Deg9 reveals a novel octameric-type HtrA protease. / Ouyang, Min; Li, Xiaoyi; Zhao, Shun; Pu, Hua; Shen, Jian-Ren; Adam, Zach; Clausen, Tim; Zhang, Lixin.

In: Nature Plants, Vol. 3, No. 12, 01.12.2017, p. 973-982.

Research output: Contribution to journalArticle

Ouyang, M, Li, X, Zhao, S, Pu, H, Shen, J-R, Adam, Z, Clausen, T & Zhang, L 2017, 'The crystal structure of Deg9 reveals a novel octameric-type HtrA protease', Nature Plants, vol. 3, no. 12, pp. 973-982. https://doi.org/10.1038/s41477-017-0060-2
Ouyang, Min ; Li, Xiaoyi ; Zhao, Shun ; Pu, Hua ; Shen, Jian-Ren ; Adam, Zach ; Clausen, Tim ; Zhang, Lixin. / The crystal structure of Deg9 reveals a novel octameric-type HtrA protease. In: Nature Plants. 2017 ; Vol. 3, No. 12. pp. 973-982.
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