The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses

Fusamichi Akita, Khoon Tee Chong, Hideaki Tanaka, Eiki Yamashita, Naoyuki Miyazaki, Yuichiro Nakaishi, Mamoru Suzuki, Kazunori Namba, Yasuko Ono, Tomitake Tsukihara, Atsushi Nakagawa

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Pyrococcus furiosus is a hyperthermophilic archaeal microorganism found near deep-sea thermal vents and its optimal growth temperature of 100 °C. Recently, a 38.8-kDa protein from P. furiosus DSM 3638 was isolated and characterized. Electron microscopy revealed that this protein aggregated as spheres of approximately 30 nm in diameter, which we designated P. furiosus virus-like particles (PfVs). X-ray crystallographic analysis at 3.6-Å resolution revealed that each PfV consisted of 180 copies of the 38.8-kDa protein and retained T = 3 icosahedral symmetry, as is often the case in spherical viruses. The total molecular mass of each particle was approximately 7 MDa. An examination of capsid structures suggested strong evolutionary links among PfV, tailed double-stranded DNA bacteriophages, and herpes viruses. The similar three-dimensional structures of the various coat proteins indicate that these viral capsids might have originated and evolved from a common ancestor. The structure of PfV provides a previously undescribed example of viral relationships across the three domains of life (Eukarya, Bacteria, and Archaea).

Original languageEnglish
Pages (from-to)1469-1483
Number of pages15
JournalJournal of Molecular Biology
Volume368
Issue number5
DOIs
Publication statusPublished - May 18 2007
Externally publishedYes

Fingerprint

Pyrococcus furiosus
Archaea
Virion
Capsid
Viruses
Hydrothermal Vents
Proteins
Capsid Proteins
Eukaryota
Bacteriophages
Electron Microscopy
X-Rays
Bacteria
Temperature
DNA
Growth

Keywords

  • hyperthermophilic Archaea
  • PfV (Pyrococcus furiosus virus-like particle)
  • Pyrococcus furiosus
  • tailed bacteriophage
  • X-ray crystallography

ASJC Scopus subject areas

  • Virology

Cite this

The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses. / Akita, Fusamichi; Chong, Khoon Tee; Tanaka, Hideaki; Yamashita, Eiki; Miyazaki, Naoyuki; Nakaishi, Yuichiro; Suzuki, Mamoru; Namba, Kazunori; Ono, Yasuko; Tsukihara, Tomitake; Nakagawa, Atsushi.

In: Journal of Molecular Biology, Vol. 368, No. 5, 18.05.2007, p. 1469-1483.

Research output: Contribution to journalArticle

Akita, F, Chong, KT, Tanaka, H, Yamashita, E, Miyazaki, N, Nakaishi, Y, Suzuki, M, Namba, K, Ono, Y, Tsukihara, T & Nakagawa, A 2007, 'The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses', Journal of Molecular Biology, vol. 368, no. 5, pp. 1469-1483. https://doi.org/10.1016/j.jmb.2007.02.075
Akita, Fusamichi ; Chong, Khoon Tee ; Tanaka, Hideaki ; Yamashita, Eiki ; Miyazaki, Naoyuki ; Nakaishi, Yuichiro ; Suzuki, Mamoru ; Namba, Kazunori ; Ono, Yasuko ; Tsukihara, Tomitake ; Nakagawa, Atsushi. / The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses. In: Journal of Molecular Biology. 2007 ; Vol. 368, No. 5. pp. 1469-1483.
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