The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis

Akiwo Tani, Chiemi Inoue, Yoko Tanaka, Yoko Yamamoto, Hideki Kondo, Syuntaro Hiradate, Kazuhide Kimbara, Fusako Kawai

Research output: Contribution to journalArticle

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Abstract

Rhodotorula glutinis IFO1125 was found to acquire increased aluminium (Al) resistance from 50 μM to more than 5 mM by repetitive culturing with stepwise increases in Al concentration at pH 4.0. To investigate the mechanism underlying this novel phenomenon, wild-type and Al-resistant cells were compared. Neither cell type accumulated the free form of Al (Al3+) added to the medium. Transmission electron microscopic analyses revealed a greater number of mitochondria in resistant cells. The formation of small mitochondria with simplified cristae structures was observed in the wild-type strain grown in the presence of Al and in resistant cells grown in the absence of Al. Addition of Al to cells resulted in high mitochondrial membrane potential and concomitant generation of reactive oxygen species (ROS). Exposure to Al also resulted in elevated levels of oxidized proteins and oxidized lipids. Addition of the antioxidants α-tocopherol and ascorbic acid alleviated the Al toxicity, suggesting that ROS generation is the main cause of Al toxicity. Differential display analysis indicated upregulation of mitochondrial genes in the resistant cells. Resistant cells were found to have 2.5- to 3-fold more mitochondrial DNA (mtDNA) than the wild-type strain. Analysis of tricarboxylic acid cycle and respiratory-chain enzyme activities in wild-type and resistant cells revealed significantly reduced cytochrome c oxidase activity and resultant high ROS production in the latter cells. Taken together, these data suggest that the adaptive increased resistance to Al stress in resistant cells resulted from an increased number of mitochondria and increased mtDNA content, as a compensatory response to reduced respiratory activity caused by a deficiency in complex IV function.

Original languageEnglish
Pages (from-to)3437-3446
Number of pages10
JournalMicrobiology
Volume154
Issue number11
DOIs
Publication statusPublished - 2008

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Rhodotorula
Aluminum
Reactive Oxygen Species
Mitochondria
Mitochondrial DNA
Cytochrome-c Oxidase Deficiency
Mitochondrial Genes
Tocopherols
Citric Acid Cycle
Mitochondrial Membrane Potential
Electron Transport Complex IV
Electron Transport
Ascorbic Acid
Up-Regulation
Antioxidants

ASJC Scopus subject areas

  • Microbiology

Cite this

The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis. / Tani, Akiwo; Inoue, Chiemi; Tanaka, Yoko; Yamamoto, Yoko; Kondo, Hideki; Hiradate, Syuntaro; Kimbara, Kazuhide; Kawai, Fusako.

In: Microbiology, Vol. 154, No. 11, 2008, p. 3437-3446.

Research output: Contribution to journalArticle

Tani, A, Inoue, C, Tanaka, Y, Yamamoto, Y, Kondo, H, Hiradate, S, Kimbara, K & Kawai, F 2008, 'The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis', Microbiology, vol. 154, no. 11, pp. 3437-3446. https://doi.org/10.1099/mic.0.2007/016048-0
Tani, Akiwo ; Inoue, Chiemi ; Tanaka, Yoko ; Yamamoto, Yoko ; Kondo, Hideki ; Hiradate, Syuntaro ; Kimbara, Kazuhide ; Kawai, Fusako. / The crucial role of mitochondrial regulation in adaptive aluminium resistance in Rhodotorula glutinis. In: Microbiology. 2008 ; Vol. 154, No. 11. pp. 3437-3446.
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