The crucial amino acid residue related to inactivation of Vibrio vulnificus hemolysin

Mitsutoshi Senoh, Yuka Okita, Sumio Shinoda, Shin-ichi Miyoshi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Vibrio vulnificus, an opportunistic human pathogen causing fetal septicemia, produces a 50-kDa pore-forming toxin as a virulence factor. This toxin consists of 451 amino acid residues; however, there are two types of this toxin on the basis of the difference of some amino acid residues, type 1 (Leu281, Ser415, Asn435/Asp435, Asn438) and type 2 (Ile281, Asn415, Asn435, Thr438). In the present study, two characteristic properties of type 2 toxin that was elaborated by V. vulnificus cells or synthesized by the in vitro system were compared to those of type 1 toxin. Type 2 toxin was found to be more resistant to spontaneous inactivation at 37 °C and to specific inactivation by cholesterol. On the other hand, a variant of type 2 toxin (Asp435, Asn438) showed the same properties as type 1 toxin. The replacement of the 438th Asn to Thr (N438T), but not the 435th Asp to Asn (D435N), resulted in reversion of the variant type 2 toxin to typical type 2 toxin. These findings indicate that a single amino acid residue, Thr438, may be critical for higher stability of type 2 toxin.

Original languageEnglish
Pages (from-to)78-83
Number of pages6
JournalMicrobial Pathogenesis
Volume44
Issue number1
DOIs
Publication statusPublished - Jan 2008

Fingerprint

Vibrio vulnificus
Amino Acids
Virulence Factors
Viperidae
Sepsis
Cholesterol
Vibrio hemolysin

Keywords

  • Hemolysin
  • In vitro translation
  • Site-directed mutagenesis
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Microbiology
  • Infectious Diseases

Cite this

The crucial amino acid residue related to inactivation of Vibrio vulnificus hemolysin. / Senoh, Mitsutoshi; Okita, Yuka; Shinoda, Sumio; Miyoshi, Shin-ichi.

In: Microbial Pathogenesis, Vol. 44, No. 1, 01.2008, p. 78-83.

Research output: Contribution to journalArticle

Senoh, Mitsutoshi ; Okita, Yuka ; Shinoda, Sumio ; Miyoshi, Shin-ichi. / The crucial amino acid residue related to inactivation of Vibrio vulnificus hemolysin. In: Microbial Pathogenesis. 2008 ; Vol. 44, No. 1. pp. 78-83.
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