The conformational polymorphism of the green fluorescent protein

Haidong Tan, Yueguang Li, Ling Chen, Takayuki Kudoh, Tomonari Kasai, Masaharu Seno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel, indicating that GST was in many different states while GFPuv and GST-GFPuv were only in four and two slightly different states. Four different circular dichroism spectra were obtained from the GFPuv polymorphisms. The single molecular behavior of GST-GFPuv and GFPuv was also characterized by MALDI-TOF MS. Thus, we demonstrated that: (1) there might be four different structural polymorphisms for the native GFPuv; (2) GFPuv could reduce its partner's polymorphism as a fusion protein. Although GFPuv had many merits as a reporter, its unreliability was found in the study.

Original languageEnglish
Pages (from-to)142-148
Number of pages7
JournalMolecular Biology
Volume46
Issue number1
DOIs
Publication statusPublished - Feb 2012

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Green Fluorescent Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Circular Dichroism
Glutathione Transferase
Proteins
polyacrylamide gels

Keywords

  • circular dichroism spectra
  • conformational polymorphism
  • glutathione-S-transferase
  • green fluorescent protein
  • MALDI-TOF MS
  • native polyacrylamide gel electrophoresis

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology

Cite this

The conformational polymorphism of the green fluorescent protein. / Tan, Haidong; Li, Yueguang; Chen, Ling; Kudoh, Takayuki; Kasai, Tomonari; Seno, Masaharu.

In: Molecular Biology, Vol. 46, No. 1, 02.2012, p. 142-148.

Research output: Contribution to journalArticle

Tan, Haidong ; Li, Yueguang ; Chen, Ling ; Kudoh, Takayuki ; Kasai, Tomonari ; Seno, Masaharu. / The conformational polymorphism of the green fluorescent protein. In: Molecular Biology. 2012 ; Vol. 46, No. 1. pp. 142-148.
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