The conformational polymorphism of the green fluorescent protein.

Haidong Tan, Yueguang Li, Ling Chen, Takayuki Kudoh, Tomonari Kasai, Masaharu Seno

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Green fluorescent protein (GFPuv) has been widely used as a reporter fused to individual targeting sequences. However, its state in liquid and its effect on other proteins are still unclear. The conformational polymorphisms of glutathione-S-transferase-green fluorescent protein (GST-GFPuv), GFPuv and GST were analyzed by native polyacrylamide gel, indicating that GST was in many different states while GFPuv and GST-GFPuv were only in four and two slightly different states. Four different circular dichroism spectra were obtained from the GFPuv polymorphisms. The single molecular behavior of GST-GFPuv and GFPuv was also characterized by MALDI-TOF MS. Thus, we demonstrated that: (1) there might be four different structural polymorphisms for the native GFPuv; (2) GFPuv could reduce its partner's polymorphism as a fusion protein. Although GFPuv had many merits as a reporter, its unreliability was found in the study.

Original languageEnglish
Pages (from-to)156-161
Number of pages6
JournalMolekuliarnaia biologiia
Volume46
Issue number1
Publication statusPublished - Jan 2012
Externally publishedYes

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Green Fluorescent Proteins
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Circular Dichroism
Glutathione Transferase
Proteins
polyacrylamide gels

ASJC Scopus subject areas

  • Molecular Biology

Cite this

The conformational polymorphism of the green fluorescent protein. / Tan, Haidong; Li, Yueguang; Chen, Ling; Kudoh, Takayuki; Kasai, Tomonari; Seno, Masaharu.

In: Molekuliarnaia biologiia, Vol. 46, No. 1, 01.2012, p. 156-161.

Research output: Contribution to journalArticle

Tan, H, Li, Y, Chen, L, Kudoh, T, Kasai, T & Seno, M 2012, 'The conformational polymorphism of the green fluorescent protein.', Molekuliarnaia biologiia, vol. 46, no. 1, pp. 156-161.
Tan, Haidong ; Li, Yueguang ; Chen, Ling ; Kudoh, Takayuki ; Kasai, Tomonari ; Seno, Masaharu. / The conformational polymorphism of the green fluorescent protein. In: Molekuliarnaia biologiia. 2012 ; Vol. 46, No. 1. pp. 156-161.
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