The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice

Mai Suzuki, Hirokazu Tanaka, Akira Tanimura, Kenji Tanabe, Natsuko Oe, Shinya Rai, Syunsuke Kon, Manabu Fukumoto, Kohji Takei, Takaya Abe, Itaru Matsumura, Yuzuru Kanakura, Toshio Watanabe

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Phosphatidylinositol binding clathrin assembly protein (PICALM), also known as clathrin assembly lymphoid myeloid leukemia protein (CALM), was originally isolated as part of the fusion gene CALM/AF10, which results from the chromosomal translocation t(10;11)(p13;q14). CALM is sufficient to drive clathrin assembly in vitro on lipid monolayers and regulates clathrin-coated budding and the size and shape of the vesicles at the plasma membrane. However, the physiological role of CALM has yet to be elucidated. Here, the role of CALM in vivo was investigated using CALM-deficient mice. CALM-deficient mice exhibited retarded growth in utero and were dwarfed throughout their shortened life-spans. Moreover, CALM-deficient mice suffered from severe anemia, and the maturation and iron content in erythroid precursors were severely impaired. CALM-deficient erythroid cells and embryonic fibroblasts exhibited impaired clathrin-mediated endocytosis of transferrin. These results indicate that CALM is required for erythroid maturation and transferrin internalization in mice.

Original languageEnglish
Article numbere31854
JournalPLoS One
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 21 2012

Fingerprint

Vesicular Transport Adaptor Proteins
Myeloid-Lymphoid Leukemia Protein
clathrin
Clathrin
transferrin
Transferrin
myeloid leukemia
mice
proteins
Genetic Translocation
Erythroid Cells
Gene Fusion
Phosphatidylinositols
Endocytosis
Anemia
gene fusion
Carrier Proteins
Fibroblasts
Cell membranes
phosphatidylinositols

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Suzuki, M., Tanaka, H., Tanimura, A., Tanabe, K., Oe, N., Rai, S., ... Watanabe, T. (2012). The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice. PLoS One, 7(2), [e31854]. https://doi.org/10.1371/journal.pone.0031854

The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice. / Suzuki, Mai; Tanaka, Hirokazu; Tanimura, Akira; Tanabe, Kenji; Oe, Natsuko; Rai, Shinya; Kon, Syunsuke; Fukumoto, Manabu; Takei, Kohji; Abe, Takaya; Matsumura, Itaru; Kanakura, Yuzuru; Watanabe, Toshio.

In: PLoS One, Vol. 7, No. 2, e31854, 21.02.2012.

Research output: Contribution to journalArticle

Suzuki, M, Tanaka, H, Tanimura, A, Tanabe, K, Oe, N, Rai, S, Kon, S, Fukumoto, M, Takei, K, Abe, T, Matsumura, I, Kanakura, Y & Watanabe, T 2012, 'The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice', PLoS One, vol. 7, no. 2, e31854. https://doi.org/10.1371/journal.pone.0031854
Suzuki, Mai ; Tanaka, Hirokazu ; Tanimura, Akira ; Tanabe, Kenji ; Oe, Natsuko ; Rai, Shinya ; Kon, Syunsuke ; Fukumoto, Manabu ; Takei, Kohji ; Abe, Takaya ; Matsumura, Itaru ; Kanakura, Yuzuru ; Watanabe, Toshio. / The clathrin assembly protein PICALM is required for erythroid maturation and transferrin internalization in mice. In: PLoS One. 2012 ; Vol. 7, No. 2.
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