The circadian clock network in the brain of different Drosophila species

Christiane Hermann, Rachele Saccon, Pingkalai R. Senthilan, Lilith Domnik, Heinrich Dircksen, Taishi Yoshii, Charlotte Helfrich-Förster

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Comparative studies on cellular and molecular clock mechanisms have revealed striking similarities in the organization of the clocks among different animal groups. To gain evolutionary insight into the properties of the clock network within the Drosophila genus, we analyzed sequence identities and similarities of clock protein homologues and immunostained brains of 10 different Drosophila species using antibodies against vrille (VRI), PAR-protein domain1 (PDP1), and cryptochrome (CRY). We found that the clock network of both subgenera Sophophora and Drosophila consists of all lateral and dorsal clock neuron clusters that were previously described in Drosophila melanogaster. Immunostaining against CRY and the neuropeptide pigment-dispersing factor (PDF), however, revealed species-specific differences. All species of the Drosophila subgenus and D. pseudoobscura of the Sophophora subgenus completely lacked CRY in the large ventrolateral clock neurons (lLNvs) and showed reduced PDF immunostaining in the small ventrolateral clock neurons (sLNvs). In contrast, we found the expression of the ion transport peptide (ITP) to be consistent within the fifth sLNv and one dorsolateral clock neuron (LNd) in all investigated species, suggesting a conserved putative function of this neuropeptide in the clock. We conclude that the general anatomy of the clock network is highly conserved throughout the Drosophila genus, although there is variation in PDF and CRY expression. Our comparative study is a first step toward understanding the organization of the circadian clock in Drosophila species adapted to different habitats.

Original languageEnglish
Pages (from-to)367-388
Number of pages22
JournalJournal of Comparative Neurology
Volume521
Issue number2
DOIs
Publication statusPublished - Feb 1 2013

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Circadian Clocks
Cryptochromes
Drosophila
Brain
Neurons
Neuropeptides
Ion Transport
Drosophila melanogaster
Ecosystem
Anatomy
Proteins
Peptides
Antibodies

Keywords

  • Circadian
  • Clock neurons
  • Cryptochrome
  • Immunostaining
  • Neuropeptide

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Hermann, C., Saccon, R., Senthilan, P. R., Domnik, L., Dircksen, H., Yoshii, T., & Helfrich-Förster, C. (2013). The circadian clock network in the brain of different Drosophila species. Journal of Comparative Neurology, 521(2), 367-388. https://doi.org/10.1002/cne.23178

The circadian clock network in the brain of different Drosophila species. / Hermann, Christiane; Saccon, Rachele; Senthilan, Pingkalai R.; Domnik, Lilith; Dircksen, Heinrich; Yoshii, Taishi; Helfrich-Förster, Charlotte.

In: Journal of Comparative Neurology, Vol. 521, No. 2, 01.02.2013, p. 367-388.

Research output: Contribution to journalArticle

Hermann, C, Saccon, R, Senthilan, PR, Domnik, L, Dircksen, H, Yoshii, T & Helfrich-Förster, C 2013, 'The circadian clock network in the brain of different Drosophila species', Journal of Comparative Neurology, vol. 521, no. 2, pp. 367-388. https://doi.org/10.1002/cne.23178
Hermann, Christiane ; Saccon, Rachele ; Senthilan, Pingkalai R. ; Domnik, Lilith ; Dircksen, Heinrich ; Yoshii, Taishi ; Helfrich-Förster, Charlotte. / The circadian clock network in the brain of different Drosophila species. In: Journal of Comparative Neurology. 2013 ; Vol. 521, No. 2. pp. 367-388.
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