The chloroplast ycf8 open reading frame encodes a photosystem II polypeptide which maintains photosynthetic activity under adverse growth conditions

Caroline Monod, Yuichiro Takahashi, Michel Goldschmidt-Clermont, Jean David Rochaix

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

We have engineered and analyzed a chloroplast mutant of Chlamydomonas reinhardtii that lacks ycf8, the chloroplast open reading frame 8, which is highly conserved in location and predicted amino acid sequence in land plants and C.reinhardtii. The ycf8 sequence was replaced with the aadA cassette which confers resistance to spectinomycin when expressed in the chloroplast. Although the mutant is able to grow phototrophically, photosystem II function and cell growth are impaired under stress conditions such as high light intensity and diminished chloroplast protein synthesis induced by spectinomycin. Use of an antibody generated against the ycf8 product has revealed that this hydrophobic polypeptide is associated with photosystem II, based on its severely reduced levels in various photosystem II-deficient mutants and on its copurification with photosystem II. This protein, therefore, appears to be (i) a novel photosystem II subunit and (ii) required for maintaining optimal photosystem II activity under adverse growth conditions.

Original languageEnglish
Pages (from-to)2747-2754
Number of pages8
JournalEMBO Journal
Volume13
Issue number12
Publication statusPublished - 1994

Fingerprint

Photosystem II Protein Complex
Chloroplasts
Open Reading Frames
Peptides
Growth
Spectinomycin
High intensity light
Embryophyta
Chloroplast Proteins
Chlamydomonas reinhardtii
Cell growth
Amino Acid Sequence
Light
Amino Acids
Antibodies
Proteins

Keywords

  • Chloroplast open reading frame
  • Photosystem II
  • Stress

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

The chloroplast ycf8 open reading frame encodes a photosystem II polypeptide which maintains photosynthetic activity under adverse growth conditions. / Monod, Caroline; Takahashi, Yuichiro; Goldschmidt-Clermont, Michel; Rochaix, Jean David.

In: EMBO Journal, Vol. 13, No. 12, 1994, p. 2747-2754.

Research output: Contribution to journalArticle

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