TY - JOUR
T1 - The chestnut blight fungus for studies on virus/host and virus/virus interactions
T2 - From a natural to a model host
AU - Eusebio-Cope, Ana
AU - Sun, Liying
AU - Tanaka, Toru
AU - Chiba, Sotaro
AU - Kasahara, Shin
AU - Suzuki, Nobuhiro
N1 - Funding Information:
A series of studies related to this paper was financially supported in part by Yomogi Inc. and the Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry . The authors are grateful to Drs. Hideki Kondo, Masatoki Taga, Ken-Ichi Ikeda, and Satoko Kanematsu, and current and previous lab members of the Suzuki lab for fruitful discussion. We would also like to thank Drs. Daniel Rigling, Donald L. Nuss, and Bradley I. Hillman for their generous gifts of the photographs of chestnut blight, plasmid constructs, fungal strains, and fruitful discussion. Plasmid clones carrying fluorescent protein genes were kindly provided by Dr. Roger Y. Tsien. The authors apologize to authors whose related articles have not been cited due to space limitation.
Publisher Copyright:
© 2014 Elsevier Inc.
PY - 2015/3/1
Y1 - 2015/3/1
N2 - The chestnut blight fungus, Cryphonectria parasitica, is an important plant pathogenic ascomycete. The fungus hosts a wide range of viruses and now has been established as a model filamentous fungus for studying virus/host and virus/virus interactions. This is based on the development of methods for artificial virus introduction and elimination, host genome manipulability, available host genome sequence with annotations, host mutant strains, and molecular tools. Molecular tools include sub-cellular distribution markers, gene expression reporters, and vectors with regulatable promoters that have been long available for unicellular organisms, cultured cells, individuals of animals and plants, and certain filamentous fungi. A comparison with other filamentous fungi such as Neurospora crassa has been made to establish clear advantages and disadvantages of C. parasitica as a virus host. In addition, a few recent studies on RNA silencing vs. viruses in this fungus are introduced.
AB - The chestnut blight fungus, Cryphonectria parasitica, is an important plant pathogenic ascomycete. The fungus hosts a wide range of viruses and now has been established as a model filamentous fungus for studying virus/host and virus/virus interactions. This is based on the development of methods for artificial virus introduction and elimination, host genome manipulability, available host genome sequence with annotations, host mutant strains, and molecular tools. Molecular tools include sub-cellular distribution markers, gene expression reporters, and vectors with regulatable promoters that have been long available for unicellular organisms, cultured cells, individuals of animals and plants, and certain filamentous fungi. A comparison with other filamentous fungi such as Neurospora crassa has been made to establish clear advantages and disadvantages of C. parasitica as a virus host. In addition, a few recent studies on RNA silencing vs. viruses in this fungus are introduced.
KW - Chestnut blight fungus
KW - Cryphonectria parasitica
KW - DsRNA
KW - Fungal virus
KW - Hypovirus
KW - Model organism
KW - Mycoreovirus
KW - Mycovirus
KW - RNA silencing
KW - Virus-host interaction
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U2 - 10.1016/j.virol.2014.09.024
DO - 10.1016/j.virol.2014.09.024
M3 - Article
C2 - 25454384
AN - SCOPUS:84929998627
SN - 0042-6822
VL - 477
SP - 164
EP - 175
JO - Virology
JF - Virology
ER -