The CENP-O complex requirement varies among different cell types

Naoko Kagawa, Tetsuya Hori, Yuko Hoki, Osamu Hosoya, Kimiko Tsutsui, Yumiko Saga, Takashi Sado, Tatsuo Fukagawa

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

CENP-U (CENP-50) is a component of the CENP-O complex, which includes CENP-O, CENP-P, CENP-Q, CENP-R, and CENP-U and is constitutively localized at kinetochores throughout the cell cycle in vertebrates. Although CENP-U deficiency results in some mitotic defects in chicken DT40 cells, CENP-U-deficient chicken DT40 cells are viable. To examine the functional roles of CENP-U in an organism-dependent context, we generated CENP-U-deficient mice. The CENP-U-deficient mice died during early embryogenesis (approximately E7.5). Thus, conditional CENP-U-deficient mouse ES cells were generated to analyze CENP-U-deficient phenotypes at the cell level. When CENP-U was disrupted in the mouse ES cells, all CENP-O complex proteins disappeared from kinetochores. In contrast, other kinetochore proteins were recruited in CENP-U-deficient mouse ES cells as CENP-U-deficient DT40 cells. However, the CENP-U-deficient ES cells died after exhibiting abnormal mitotic behavior. Although CENP-U was essential for cell viability during mouse early embryogenesis, CENP-U-deficient mouse embryonic fibroblast cells were viable, similar to the DT40 cells. Thus, although both DT40 and ES cells with CENP-U deficiency have similar mitotic defects, cellular responses to mitotic defects vary among different cell types.

Original languageEnglish
Pages (from-to)293-303
Number of pages11
JournalChromosome Research
Volume22
Issue number3
DOIs
Publication statusPublished - 2014

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Kinetochores
Embryonic Development
Chickens
Vertebrates
Cell Survival
Cell Cycle
Proteins
Fibroblasts
Phenotype

Keywords

  • CENP-O complex proteins
  • Centromere
  • Kinetochore

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Kagawa, N., Hori, T., Hoki, Y., Hosoya, O., Tsutsui, K., Saga, Y., ... Fukagawa, T. (2014). The CENP-O complex requirement varies among different cell types. Chromosome Research, 22(3), 293-303. https://doi.org/10.1007/s10577-014-9404-1

The CENP-O complex requirement varies among different cell types. / Kagawa, Naoko; Hori, Tetsuya; Hoki, Yuko; Hosoya, Osamu; Tsutsui, Kimiko; Saga, Yumiko; Sado, Takashi; Fukagawa, Tatsuo.

In: Chromosome Research, Vol. 22, No. 3, 2014, p. 293-303.

Research output: Contribution to journalArticle

Kagawa, N, Hori, T, Hoki, Y, Hosoya, O, Tsutsui, K, Saga, Y, Sado, T & Fukagawa, T 2014, 'The CENP-O complex requirement varies among different cell types', Chromosome Research, vol. 22, no. 3, pp. 293-303. https://doi.org/10.1007/s10577-014-9404-1
Kagawa, Naoko ; Hori, Tetsuya ; Hoki, Yuko ; Hosoya, Osamu ; Tsutsui, Kimiko ; Saga, Yumiko ; Sado, Takashi ; Fukagawa, Tatsuo. / The CENP-O complex requirement varies among different cell types. In: Chromosome Research. 2014 ; Vol. 22, No. 3. pp. 293-303.
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