The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α

D. Azakami; R. Nakahira; Y. Kato; M. Michishita; M. Kobayashi; E. Onozawa; M. Bonkobara; M. Kobayashi; K. Takahashi; M. Watanabe; K. Ishioka; T. Sako; K. Ochiai; T. Omi

doi:10.1111/vco.12199

The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α

D. Azakami, R. Nakahira, Y. Kato, M. Michishita, M. Kobayashi, E. Onozawa, M. Bonkobara, M. Kobayashi, K. Takahashi, M. Watanabe, K. Ishioka, T. Sako, K. Ochiai, T. Omi

University Hospital

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there are only a few tools for analysing canine prostate cancer by using a cell biological approach. Therefore, to evaluate androgen-independent neoplastic cell growth, a new canine prostate cancer cell line (CHP-1) was established in this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is over-expressed in human androgen-independent prostate cancer. The CHP-1 xenograft also showed SGTA over-expression. Although CHP-1 shows poor androgen receptor (AR) signalling upon dihydrotestosterone stimulation, forced expression of AR enabled evaluation of AR signalling. Taken together, these results suggest that CHP-1 will be a useful model for investigating the pathogenesis of androgen-dependent and androgen-independent canine prostate cancer.

Original language	English
Pages (from-to)	557-562
Number of pages	6
Journal	Veterinary and Comparative Oncology
Volume	15
Issue number	2
DOIs	https://doi.org/10.1111/vco.12199
Publication status	Published - Jun 2017

Keywords

androgen receptor signalling
canine
prostate cancer
small glutamine-rich tetratricopeptide repeat-containing protein α

ASJC Scopus subject areas

veterinary(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/vco.12199

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Azakami, D., Nakahira, R., Kato, Y., Michishita, M., Kobayashi, M., Onozawa, E., Bonkobara, M., Kobayashi, M., Takahashi, K., Watanabe, M., Ishioka, K., Sako, T., Ochiai, K., & Omi, T. (2017). The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α. Veterinary and Comparative Oncology, 15(2), 557-562. https://doi.org/10.1111/vco.12199

Azakami, D, Nakahira, R, Kato, Y, Michishita, M, Kobayashi, M, Onozawa, E, Bonkobara, M, Kobayashi, M, Takahashi, K, Watanabe, M, Ishioka, K, Sako, T, Ochiai, K & Omi, T 2017, 'The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α', Veterinary and Comparative Oncology, vol. 15, no. 2, pp. 557-562. https://doi.org/10.1111/vco.12199

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abstract = "Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there are only a few tools for analysing canine prostate cancer by using a cell biological approach. Therefore, to evaluate androgen-independent neoplastic cell growth, a new canine prostate cancer cell line (CHP-1) was established in this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is over-expressed in human androgen-independent prostate cancer. The CHP-1 xenograft also showed SGTA over-expression. Although CHP-1 shows poor androgen receptor (AR) signalling upon dihydrotestosterone stimulation, forced expression of AR enabled evaluation of AR signalling. Taken together, these results suggest that CHP-1 will be a useful model for investigating the pathogenesis of androgen-dependent and androgen-independent canine prostate cancer.",

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author = "D. Azakami and R. Nakahira and Y. Kato and M. Michishita and M. Kobayashi and E. Onozawa and M. Bonkobara and M. Kobayashi and K. Takahashi and M. Watanabe and K. Ishioka and T. Sako and K. Ochiai and T. Omi",

note = "Funding Information: This work was supported by KAKENHI scientific research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Nos. 15K07754, 26292161, and 26450400). Publisher Copyright: {\textcopyright} 2016 John Wiley & Sons Ltd",

year = "2017",

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doi = "10.1111/vco.12199",

language = "English",

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pages = "557--562",

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AU - Azakami, D.

AU - Nakahira, R.

AU - Kato, Y.

AU - Michishita, M.

AU - Kobayashi, M.

AU - Onozawa, E.

AU - Bonkobara, M.

AU - Kobayashi, M.

AU - Takahashi, K.

AU - Watanabe, M.

AU - Ishioka, K.

AU - Sako, T.

AU - Ochiai, K.

AU - Omi, T.

N1 - Funding Information: This work was supported by KAKENHI scientific research grants from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Nos. 15K07754, 26292161, and 26450400). Publisher Copyright: © 2016 John Wiley & Sons Ltd

PY - 2017/6

Y1 - 2017/6

N2 - Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there are only a few tools for analysing canine prostate cancer by using a cell biological approach. Therefore, to evaluate androgen-independent neoplastic cell growth, a new canine prostate cancer cell line (CHP-1) was established in this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is over-expressed in human androgen-independent prostate cancer. The CHP-1 xenograft also showed SGTA over-expression. Although CHP-1 shows poor androgen receptor (AR) signalling upon dihydrotestosterone stimulation, forced expression of AR enabled evaluation of AR signalling. Taken together, these results suggest that CHP-1 will be a useful model for investigating the pathogenesis of androgen-dependent and androgen-independent canine prostate cancer.

AB - Although androgen therapy resistance and poor clinical outcomes are seen in most canine prostate cancer cases, there are only a few tools for analysing canine prostate cancer by using a cell biological approach. Therefore, to evaluate androgen-independent neoplastic cell growth, a new canine prostate cancer cell line (CHP-1) was established in this study. CHP-1 over-expressed the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α (SGTA), which is over-expressed in human androgen-independent prostate cancer. The CHP-1 xenograft also showed SGTA over-expression. Although CHP-1 shows poor androgen receptor (AR) signalling upon dihydrotestosterone stimulation, forced expression of AR enabled evaluation of AR signalling. Taken together, these results suggest that CHP-1 will be a useful model for investigating the pathogenesis of androgen-dependent and androgen-independent canine prostate cancer.

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The canine prostate cancer cell line CHP-1 shows over-expression of the co-chaperone small glutamine-rich tetratricopeptide repeat-containing protein α

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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