The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production

Wenxin Tang, Lili Tu, Xiyan Yang, Jiafu Tan, Fenglin Deng, Juan Hao, Kai Guo, Keith Lindsey, Xianlong Zhang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Fiber elongation is the key determinant of fiber quality and output in cotton (Gossypium hirsutum). Although expression profiling and functional genomics provide some data, the mechanism of fiber development is still not well understood. Here, a gene encoding a calcium sensor, GhCaM7, was isolated based on its high expression level relative to other GhCaMs in fiber cells at the fast elongation stage. The level of expression of GhCaM7 in the wild-type and the fuzzless/lintless mutant correspond to the presence and absence, respectively, of fiber initials. Overexpressing GhCaM7 promotes early fiber elongation, whereas GhCaM7 suppression by RNAi delays fiber initiation and inhibits fiber elongation. Reactive oxygen species (ROS) play important roles in early fiber development. ROS induced by exogenous hydrogen peroxide (H2O2) and Ca2+ starvation promotes early fiber elongation. GhCaM7 overexpression fiber cells show increased ROS concentrations compared with the wild-type, while GhCaM7 RNAi fiber cells have reduced concentrations. Furthermore, we show that H2O2 enhances Ca2+ influx into the fiber and feedback-regulates the expression of GhCaM7. We conclude that GhCaM7, Ca2+ and ROS are three important regulators involved in early fiber elongation. GhCaM7 might modulate ROS production and act as a molecular link between Ca2+ and ROS signal pathways in early fiber development.

Original languageEnglish
Pages (from-to)509-520
Number of pages12
JournalNew Phytologist
Volume202
Issue number2
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Cotton Fiber
lint cotton
reactive oxygen species
Reactive Oxygen Species
Calcium
calcium
fiber cells
RNA Interference
Gossypium
fiber quality
Starvation
Genomics
Gossypium hirsutum
Hydrogen Peroxide
starvation
hydrogen peroxide
signal transduction
Signal Transduction
cotton
genomics

Keywords

  • Calcium signaling
  • Calmodulin
  • Feedback regulation
  • Fiber elongation
  • Gossypium hirsutum (cotton)
  • Reactive oxygen species (ROS)

ASJC Scopus subject areas

  • Plant Science
  • Physiology

Cite this

The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production. / Tang, Wenxin; Tu, Lili; Yang, Xiyan; Tan, Jiafu; Deng, Fenglin; Hao, Juan; Guo, Kai; Lindsey, Keith; Zhang, Xianlong.

In: New Phytologist, Vol. 202, No. 2, 2014, p. 509-520.

Research output: Contribution to journalArticle

Tang, W, Tu, L, Yang, X, Tan, J, Deng, F, Hao, J, Guo, K, Lindsey, K & Zhang, X 2014, 'The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production', New Phytologist, vol. 202, no. 2, pp. 509-520. https://doi.org/10.1111/nph.12676
Tang, Wenxin ; Tu, Lili ; Yang, Xiyan ; Tan, Jiafu ; Deng, Fenglin ; Hao, Juan ; Guo, Kai ; Lindsey, Keith ; Zhang, Xianlong. / The calcium sensor GhCaM7 promotes cotton fiber elongation by modulating reactive oxygen species (ROS) production. In: New Phytologist. 2014 ; Vol. 202, No. 2. pp. 509-520.
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