The calcium activation of gelsolin: Insights from the 3 Å structure of the G4-G6/actin complex

Han Choe, Leslie D. Burtnick, Marisan Mejillano, Helen L. Yin, Robert C. Robinson, Senyon Choe

Research output: Contribution to journalArticle

99 Citations (Scopus)

Abstract

Gelsolin participates in the reorganization of the actin cytoskeleton that is required during such phenomena as cell movement, cytokinesis, and apoptosis. It consists of six structurally similar domains, G1-G6, which are arranged at resting intracellular levels of calcium ion so as to obscure the three actin-binding surfaces. Elevation of Ca2+ concentrations releases latches within the constrained structure and produces large shifts in the relative positioning of the domains, permitting gelsolin to bind to and sever actin filaments. How Ca2+ is able to activate gelsolin has been a major question concerning the function of this protein. We present the improved structure of the C-terminal half of gelsolin bound to monomeric actin at 3.0 Å resolution. Two classes of Ca2+-binding site are evident on gelsolin: type 1 sites share coordination of Ca2+ with actin, while type 2 sites are wholly contained within gelsolin. This structure of the complex reveals the locations of two novel metal ion-binding sites in domains G5 and G6, respectively. We identify both as type 2 sites. The absolute conservation of the type 2 calcium-ligating residues across the six domains of gelsolin suggests that this site exists in each of the domains. In total, gelsolin has the potential to bind eight calcium ions, two type 1 and six type 2. The function of the type 2 sites is to facilitate structural rearrangements within gelsolin as part of the activation and actin-binding and severing processes. We propose the novel type 2 site in G6 to be the critical site that initiates overall activation of gelsolin by releasing the tail latch that locks calcium-free gelsolin in a conformation unable to bind actin.

Original languageEnglish
Pages (from-to)691-702
Number of pages12
JournalJournal of Molecular Biology
Volume324
Issue number4
DOIs
Publication statusPublished - Jan 1 2002
Externally publishedYes

Fingerprint

Gelsolin
Actins
Calcium
Ions
Actin Cytoskeleton
Binding Sites
Cytokinesis
Cell Movement

Keywords

  • Actin
  • Activation
  • Calcium
  • Gelsolin
  • Severing

ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

Cite this

The calcium activation of gelsolin : Insights from the 3 Å structure of the G4-G6/actin complex. / Choe, Han; Burtnick, Leslie D.; Mejillano, Marisan; Yin, Helen L.; Robinson, Robert C.; Choe, Senyon.

In: Journal of Molecular Biology, Vol. 324, No. 4, 01.01.2002, p. 691-702.

Research output: Contribution to journalArticle

Choe, Han ; Burtnick, Leslie D. ; Mejillano, Marisan ; Yin, Helen L. ; Robinson, Robert C. ; Choe, Senyon. / The calcium activation of gelsolin : Insights from the 3 Å structure of the G4-G6/actin complex. In: Journal of Molecular Biology. 2002 ; Vol. 324, No. 4. pp. 691-702.
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AB - Gelsolin participates in the reorganization of the actin cytoskeleton that is required during such phenomena as cell movement, cytokinesis, and apoptosis. It consists of six structurally similar domains, G1-G6, which are arranged at resting intracellular levels of calcium ion so as to obscure the three actin-binding surfaces. Elevation of Ca2+ concentrations releases latches within the constrained structure and produces large shifts in the relative positioning of the domains, permitting gelsolin to bind to and sever actin filaments. How Ca2+ is able to activate gelsolin has been a major question concerning the function of this protein. We present the improved structure of the C-terminal half of gelsolin bound to monomeric actin at 3.0 Å resolution. Two classes of Ca2+-binding site are evident on gelsolin: type 1 sites share coordination of Ca2+ with actin, while type 2 sites are wholly contained within gelsolin. This structure of the complex reveals the locations of two novel metal ion-binding sites in domains G5 and G6, respectively. We identify both as type 2 sites. The absolute conservation of the type 2 calcium-ligating residues across the six domains of gelsolin suggests that this site exists in each of the domains. In total, gelsolin has the potential to bind eight calcium ions, two type 1 and six type 2. The function of the type 2 sites is to facilitate structural rearrangements within gelsolin as part of the activation and actin-binding and severing processes. We propose the novel type 2 site in G6 to be the critical site that initiates overall activation of gelsolin by releasing the tail latch that locks calcium-free gelsolin in a conformation unable to bind actin.

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