The C-terminal domain promotes the hemorrhagic damage caused by Vibrio vulnificus metalloprotease

Shin-ichi Miyoshi, K. Kawata, K. Tomochika, S. Shinoda, S. Yamamoto

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Vibrio vulnificus, an opportunistic human pathogen, produces a 45-kDa zinc metalloprotease (V. vulnificus protease; VVP) as an important virulence determinant. VVP injected intradermally into the dorsal skin causes the hemorrhagic damage through specific degradation of type IV collage in the vascular basement membrane. The N-terminal 35-kDa polypeptide (VVP-N), the catalytic domain, also evoked the hemorrhagic skin reaction within minutes. However, the hemorrhagic activity of VVP-N was one-third of that of VVP. Besides, the proteolytic activity of VVP-N toward the reconstituted basement membrane or type IV collagen was found to be about 50% of VVP. VVP-N, like VVP, was quickly inactivated by an equimolar amount of α2-macroglobulin, a broad-spectrum plasma protease inhibitor. These findings indicate that the C-terminal 10-kDa polypeptide, the substrate-binding domain mediating the effective binding to protein substrates, functions to augment the hemorrhagic reaction of VVP.

Original languageEnglish
Pages (from-to)1883-1886
Number of pages4
JournalToxicon
Volume39
Issue number12
DOIs
Publication statusPublished - 2001

Fingerprint

Vibrio vulnificus
Metalloproteases
Skin
Macroglobulins
Peptides
Collagen Type IV
Pathogens
Substrates
Protease Inhibitors
Basement Membrane
Blood Vessels
Virulence
Zinc
Catalytic Domain
Carrier Proteins
Peptide Hydrolases
Plasmas
Degradation
Proteins

Keywords

  • Functional domain
  • Hemorrhage
  • Protease
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Toxicology

Cite this

The C-terminal domain promotes the hemorrhagic damage caused by Vibrio vulnificus metalloprotease. / Miyoshi, Shin-ichi; Kawata, K.; Tomochika, K.; Shinoda, S.; Yamamoto, S.

In: Toxicon, Vol. 39, No. 12, 2001, p. 1883-1886.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Kawata, K. ; Tomochika, K. ; Shinoda, S. ; Yamamoto, S. / The C-terminal domain promotes the hemorrhagic damage caused by Vibrio vulnificus metalloprotease. In: Toxicon. 2001 ; Vol. 39, No. 12. pp. 1883-1886.
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