The brain link protein-1 (BRAL1)

cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain

Satoshi Hirakawa, Toshitaka Oohashi, Wei Dong Su, Hidekatsu Yoshioka, Takuro Murakami, Jirô Arata, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

We report here molecular cloning and expression analysis of the gene for a novel human brain link protein-1 (BRAL1) which is predominantly expressed in brain. The predicted open reading frame of human brain link protein-1 encoded a polypeptide of 340 amino acids containing three protein modules, the immunoglobulin-like fold and proteoglycan tandem repeat 1 and 2 domains, with an estimated mass of 38 kDa. The brain link protein-1 mRNA was exclusively present in brain. When analyzed during mouse development, it was detected solely in the adult brain. Concomitant expression pattern of mRNAs for brain link protein-1 and various lectican proteoglycans in brain suggests a possibility that brain link protein-1 functions to stabilize the binding between hyaluronan and brevican. The human BRAL1 gene contained 7 exons and spanned ~6 kb. The entire immunoglobulin-like fold was encoded by a single exon and the proteoglycan tandem repeat 1 and 2 domains were encoded by a single and two exons, respectively. The deduced amino acid sequence of human brain link protein-1 exhibited 45% identity with human cartilage link protein-1 (CRTL1), previously reported as link protein to stabilize aggregates of aggrecan and hyaluronan in cartilage. These results suggest that brain link protein-1 may have distinct function from cartilage link protein-1 and play specific roles, especially in the adult brain. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)982-989
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume276
Issue number3
DOIs
Publication statusPublished - Oct 5 2000

Fingerprint

Cloning
Organism Cloning
Brain
Complementary DNA
Proteoglycans
Exons
Tandem Repeat Sequences
Hyaluronic Acid
Brevican
Immunoglobulins
Genes
link protein
Aggrecans
Amino Acids
Messenger RNA
Cartilage
Molecular Cloning
Open Reading Frames
Amino Acid Sequence
Gene Expression

Keywords

  • Brain
  • Hyaluronan
  • Lectican proteoglycan
  • Link protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

The brain link protein-1 (BRAL1) : cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. / Hirakawa, Satoshi; Oohashi, Toshitaka; Su, Wei Dong; Yoshioka, Hidekatsu; Murakami, Takuro; Arata, Jirô; Ninomiya, Yoshifumi.

In: Biochemical and Biophysical Research Communications, Vol. 276, No. 3, 05.10.2000, p. 982-989.

Research output: Contribution to journalArticle

Hirakawa, Satoshi ; Oohashi, Toshitaka ; Su, Wei Dong ; Yoshioka, Hidekatsu ; Murakami, Takuro ; Arata, Jirô ; Ninomiya, Yoshifumi. / The brain link protein-1 (BRAL1) : cDNA cloning, genomic structure, and characterization as a novel link protein expressed in adult brain. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 276, No. 3. pp. 982-989.
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