The bovine fibroblast growth factor receptor 3 (FGFR3) gene is not the locus responsible for bovine chondrodysplastic dwarfism in Japanese brown cattle

M. Takami, K. Yoneda, Y. Kobayashi, Y. Moritomo, S. R. Kata, J. E. Womack, Tetsuo Kunieda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Fibroblast growth factor receptor 3 (FGFR3) is one of the four distinct membrane-spanning tyrosine kinase receptors for fibroblast growth factors. The FGFR3 is a negative regulator of endochondral ossification and mutations in the FGFR3 gene have been found in patients of human hereditary diseases with chondrodysplastic phenotypes. Recently, we mapped the locus responsible for hereditary chondrodysplastic dwarfism in Japanese brown cattle to the distal region of bovine chromosome 6 close to the FGFR3 gene, suggesting that FGFR3 was a positional candidate gene for this disorder. In the present study, we isolated complementary DNA (cDNA) clones containing the entire coding region of the bovine FGFR3 gene. Comparison of the nucleotide sequence between affected and normal animals revealed no disease-specific differences in the deduced amino acid sequences. We further refined the localization of FGFR3 by radiation hybrid mapping, which is distinct from that of the disease locus. Therefore we conclude that bovine chondrodysplastic dwarfism in Japanese brown cattle is not caused by mutation in the FGFR3 gene.

Original languageEnglish
Pages (from-to)351-355
Number of pages5
JournalAnimal Genetics
Volume33
Issue number5
DOIs
Publication statusPublished - Oct 2002

Fingerprint

Receptor, Fibroblast Growth Factor, Type 3
Dwarfism
Wagyu
dwarfing
loci
cattle
Genes
genes
Radiation Hybrid Mapping
mutation
Mutation
Inborn Genetic Diseases
Chromosomes, Human, Pair 6
complementary DNA
Fibroblast Growth Factors
fibroblast growth factor receptor 3
Receptor Protein-Tyrosine Kinases
genetic disorders
bone formation
Osteogenesis

Keywords

  • Cattle
  • Chondrodysplasia
  • FGFR3
  • RH mapping

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Genetics(clinical)

Cite this

The bovine fibroblast growth factor receptor 3 (FGFR3) gene is not the locus responsible for bovine chondrodysplastic dwarfism in Japanese brown cattle. / Takami, M.; Yoneda, K.; Kobayashi, Y.; Moritomo, Y.; Kata, S. R.; Womack, J. E.; Kunieda, Tetsuo.

In: Animal Genetics, Vol. 33, No. 5, 10.2002, p. 351-355.

Research output: Contribution to journalArticle

Takami, M. ; Yoneda, K. ; Kobayashi, Y. ; Moritomo, Y. ; Kata, S. R. ; Womack, J. E. ; Kunieda, Tetsuo. / The bovine fibroblast growth factor receptor 3 (FGFR3) gene is not the locus responsible for bovine chondrodysplastic dwarfism in Japanese brown cattle. In: Animal Genetics. 2002 ; Vol. 33, No. 5. pp. 351-355.
@article{d2e39c2447c346f7acbafa5d86208b5b,
title = "The bovine fibroblast growth factor receptor 3 (FGFR3) gene is not the locus responsible for bovine chondrodysplastic dwarfism in Japanese brown cattle",
abstract = "Fibroblast growth factor receptor 3 (FGFR3) is one of the four distinct membrane-spanning tyrosine kinase receptors for fibroblast growth factors. The FGFR3 is a negative regulator of endochondral ossification and mutations in the FGFR3 gene have been found in patients of human hereditary diseases with chondrodysplastic phenotypes. Recently, we mapped the locus responsible for hereditary chondrodysplastic dwarfism in Japanese brown cattle to the distal region of bovine chromosome 6 close to the FGFR3 gene, suggesting that FGFR3 was a positional candidate gene for this disorder. In the present study, we isolated complementary DNA (cDNA) clones containing the entire coding region of the bovine FGFR3 gene. Comparison of the nucleotide sequence between affected and normal animals revealed no disease-specific differences in the deduced amino acid sequences. We further refined the localization of FGFR3 by radiation hybrid mapping, which is distinct from that of the disease locus. Therefore we conclude that bovine chondrodysplastic dwarfism in Japanese brown cattle is not caused by mutation in the FGFR3 gene.",
keywords = "Cattle, Chondrodysplasia, FGFR3, RH mapping",
author = "M. Takami and K. Yoneda and Y. Kobayashi and Y. Moritomo and Kata, {S. R.} and Womack, {J. E.} and Tetsuo Kunieda",
year = "2002",
month = "10",
doi = "10.1046/j.1365-2052.2002.00881.x",
language = "English",
volume = "33",
pages = "351--355",
journal = "Animal Genetics",
issn = "0268-9146",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - The bovine fibroblast growth factor receptor 3 (FGFR3) gene is not the locus responsible for bovine chondrodysplastic dwarfism in Japanese brown cattle

AU - Takami, M.

AU - Yoneda, K.

AU - Kobayashi, Y.

AU - Moritomo, Y.

AU - Kata, S. R.

AU - Womack, J. E.

AU - Kunieda, Tetsuo

PY - 2002/10

Y1 - 2002/10

N2 - Fibroblast growth factor receptor 3 (FGFR3) is one of the four distinct membrane-spanning tyrosine kinase receptors for fibroblast growth factors. The FGFR3 is a negative regulator of endochondral ossification and mutations in the FGFR3 gene have been found in patients of human hereditary diseases with chondrodysplastic phenotypes. Recently, we mapped the locus responsible for hereditary chondrodysplastic dwarfism in Japanese brown cattle to the distal region of bovine chromosome 6 close to the FGFR3 gene, suggesting that FGFR3 was a positional candidate gene for this disorder. In the present study, we isolated complementary DNA (cDNA) clones containing the entire coding region of the bovine FGFR3 gene. Comparison of the nucleotide sequence between affected and normal animals revealed no disease-specific differences in the deduced amino acid sequences. We further refined the localization of FGFR3 by radiation hybrid mapping, which is distinct from that of the disease locus. Therefore we conclude that bovine chondrodysplastic dwarfism in Japanese brown cattle is not caused by mutation in the FGFR3 gene.

AB - Fibroblast growth factor receptor 3 (FGFR3) is one of the four distinct membrane-spanning tyrosine kinase receptors for fibroblast growth factors. The FGFR3 is a negative regulator of endochondral ossification and mutations in the FGFR3 gene have been found in patients of human hereditary diseases with chondrodysplastic phenotypes. Recently, we mapped the locus responsible for hereditary chondrodysplastic dwarfism in Japanese brown cattle to the distal region of bovine chromosome 6 close to the FGFR3 gene, suggesting that FGFR3 was a positional candidate gene for this disorder. In the present study, we isolated complementary DNA (cDNA) clones containing the entire coding region of the bovine FGFR3 gene. Comparison of the nucleotide sequence between affected and normal animals revealed no disease-specific differences in the deduced amino acid sequences. We further refined the localization of FGFR3 by radiation hybrid mapping, which is distinct from that of the disease locus. Therefore we conclude that bovine chondrodysplastic dwarfism in Japanese brown cattle is not caused by mutation in the FGFR3 gene.

KW - Cattle

KW - Chondrodysplasia

KW - FGFR3

KW - RH mapping

UR - http://www.scopus.com/inward/record.url?scp=0036802040&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036802040&partnerID=8YFLogxK

U2 - 10.1046/j.1365-2052.2002.00881.x

DO - 10.1046/j.1365-2052.2002.00881.x

M3 - Article

C2 - 12354143

AN - SCOPUS:0036802040

VL - 33

SP - 351

EP - 355

JO - Animal Genetics

JF - Animal Genetics

SN - 0268-9146

IS - 5

ER -