The biological effects of antiadhesion agents on activated RAW264.7 macrophages

Toshihiro Habara, Mikiya Nakatsuka, Hideki Konishi, Kazuo Asagiri, Soichi Noguchi, Takafumi Kudohabara

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23 Citations (Scopus)


The objective of this study is to determine the biological effects of various antiadhesion agents on macrophages, which play an essential role in wound healing and adhesion. To determine these effects, RAW264.7 macrophages were activated with lipopolysaccharide in the presence of antiadhesion agents: oxidized regenerated cellulose (oxyC), sodium hyaluronate (HA), dexamethasone (Dex), or chondroitin sulfate (CS). The release of nitric oxide (NO), vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), or matrix metalloproteinases (MMPs) from RAW264.7 was measured. We found that oxyC reduced the release of NO, IL-6, MMP-2, and MMP-9, whereas it enhanced the release of VEGF. HA reduced the release of MMP-2, whereas it enhanced the release of VEGF and NO. HA exhibited no significant effect on the release of IL-6 or MMP-9. Dex reduced the release of NO, VEGF, IL-6, MMP-2, and MMP-9. CS reduced the release of VEGF, IL-6, and MMP-2, although it had no significant effect on the release of NO and MMP-9. Antiadhesion agents, which have been clinically used as physical barriers, modulated the functions of macrophages.

Original languageEnglish
Pages (from-to)628-633
Number of pages6
JournalJournal of Biomedical Materials Research
Issue number4
Publication statusPublished - Sep 15 2002



  • Antiadhesion agents
  • Cytokines
  • Macrophage
  • Metalloproteinases
  • Nitric oxide

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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