The BglII-N fragment of herpes simplex virus type 2 contains a region responsible for resistance to antiviral effects of interferon

Masaya Narita, Yasutaka Ando, Sumi Soushi, Takeshi Kurata, Yujiro Arao

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Abstract

Double infection with two interferon (IFN)-sensitive strains of herpes simplex virus (HSV), HSV-1(17syn) and HSV-2(UW268), showed reduced inhibition of virus growth by IFN. Intertypic recombinants with IFN resistance were obtained from the doubly infected cultures. These results indicate that HSV IFN resistance is controlled by at least two genetic regions. Restriction endonuclease analysis demonstrated that the recombinants were similar to HSV-2 in their genomic structure but the BamHI-A, BglII-I and BglII-N fragments of HSV-2 were commonly lost in the recombinants, suggesting that any of these fragments could be associated with HSV-2 IFN resistance. We cloned these fragments and BamHI-E, which overlaps BglII-N, from an IFN-resistant HSV-2 strain, HSV-2(G), and examined each fragment for its ability to rescue IFN resistance of HSV-2(UW268) by co-transfecting with the HSV-2(UW268) genome. Of the HSV-2(G) fragments, only BglII-N increased plating efficiency of progeny viruses in IFN-treated cells. An IFN-resistant HSV-2 clone was obtained from the BglII-N of HSV-2(G) and HSV-2(UW268) genome co-transfected culture, and a part of BglII-N of HSV-2(UW268) was replaced with that of HSV-2(G) in the HSV-2 clone. Thus, it was concluded that one of the HSV regions encoding IFN resistance is located on the BglII-N fragment of HSV-2.

Original languageEnglish
Pages (from-to)565-572
Number of pages8
JournalJournal of General Virology
Volume79
Issue number3
Publication statusPublished - Mar 1998
Externally publishedYes

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Human Herpesvirus 2
Interferons
Antiviral Agents
Simplexvirus
Clone Cells
Genome
Viruses
DNA Restriction Enzymes
Human Herpesvirus 1
Coculture Techniques

ASJC Scopus subject areas

  • Virology
  • Immunology

Cite this

The BglII-N fragment of herpes simplex virus type 2 contains a region responsible for resistance to antiviral effects of interferon. / Narita, Masaya; Ando, Yasutaka; Soushi, Sumi; Kurata, Takeshi; Arao, Yujiro.

In: Journal of General Virology, Vol. 79, No. 3, 03.1998, p. 565-572.

Research output: Contribution to journalArticle

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