The BF4 and p71 antenna mutants from Chlamydomonas reinhardtii

Sandrine Bujaldon, Natsumi Kodama, Mithun Kumar Rathod, Nicolas Tourasse, Shin Ichiro Ozawa, Julien Sellés, Olivier Vallon, Yuichiro Takahashi, Francis André Wollman

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Abstract

Two pale green mutants of the green alga Chlamydomonas reinhardtii, which have been used over the years in many photosynthesis studies, the BF4 and p71 mutants, were characterized and their mutated gene identified in the nuclear genome. The BF4 mutant is defective in the insertase Alb3.1 whereas p71 is defective in cpSRP43. The two mutants showed strikingly similar deficiencies in most of the peripheral antenna proteins associated with either photosystem I or photosystem 2. As a result the two photosystems have a reduced antenna size with photosystem 2 being the most affected. Still up to 20% of the antenna proteins remain in these strains, with the heterodimer Lhca5/Lhca6 showing a lower sensitivity to these mutations. We discuss these phenotypes in light of those of other allelic mutants that have been described in the literature and suggest that eventhough the cpSRP route serves as the main biogenesis pathway for antenna proteins, there should be an escape pathway which remains to be genetically identified.

Original languageEnglish
Article number148085
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume1861
Issue number4
DOIs
Publication statusPublished - Apr 1 2020

Keywords

  • ALB3
  • Antenna
  • Chlamydomonas
  • Light harvesting complex
  • Photosynthesis
  • cpSRP

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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  • Cite this

    Bujaldon, S., Kodama, N., Rathod, M. K., Tourasse, N., Ozawa, S. I., Sellés, J., Vallon, O., Takahashi, Y., & Wollman, F. A. (2020). The BF4 and p71 antenna mutants from Chlamydomonas reinhardtii. Biochimica et Biophysica Acta - Bioenergetics, 1861(4), [148085]. https://doi.org/10.1016/j.bbabio.2019.148085