The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia

Ryusuke Yokoyama, Taku Takahashi, Atsushi Kato, Keiko U. Torii, Yoshibumi Komeda

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In Arabidopsis thaliana (L.) Heynh, the mutation in ERECTA is known to confer a compact inflorescence by a reduction in the lengths of internodes and pedicels. We analyzed the expression pattern of this gene during plant development. In situ hybridization and histochemical analysis using transgenic plants carrying chimeric gene fusions, with the ERECTA promoter fused to the β-glucuronidase (GUS) gene, showed that ERECTA was predominantly expressed in the shoot apical meristems and organ primordia. ERECTA expression in the shoot apical meristem was weak early in plant development but increased with the transition from the vegetative to the reproductive growth phase. ERECTA was also strongly expressed in organ primordia and immature organs but weakly in mature organs. Thus, ERECTA was expressed in a cell-specific and developmentally regulated manner. In order to identify the regulatory mechanism responsible for the expression pattern of ERECTA, the cis-acting regions in the ERECTA promoter were defined by study of the expression of the chimeric genes that consist of the 5'- or internal deleted promoter and a GUS reporter gene in transgenic plants. The results showed that the essential cis-regulatory elements governing the spatially and temporally specific expression of ERECTA are located between positions -462 and -228 bp and between positions -228 and -153 bp with respect to the transcriptional initiation site.

Original languageEnglish
Pages (from-to)301-310
Number of pages10
JournalPlant Journal
Volume15
Issue number3
DOIs
Publication statusPublished - Aug 1998
Externally publishedYes

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Plant Development
Meristem
Genetically Modified Plants
Glucuronidase
shoot meristems
apical meristems
Arabidopsis
promoter regions
Gene Expression
Inflorescence
plant development
transgenic plants
Gene Fusion
Reporter Genes
Genes
In Situ Hybridization
regulatory sequences
genes
gene fusion
pedicel

ASJC Scopus subject areas

  • Plant Science

Cite this

The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia. / Yokoyama, Ryusuke; Takahashi, Taku; Kato, Atsushi; Torii, Keiko U.; Komeda, Yoshibumi.

In: Plant Journal, Vol. 15, No. 3, 08.1998, p. 301-310.

Research output: Contribution to journalArticle

Yokoyama, Ryusuke ; Takahashi, Taku ; Kato, Atsushi ; Torii, Keiko U. ; Komeda, Yoshibumi. / The Arabidopsis ERECTA gene is expressed in the shoot apical meristem and organ primordia. In: Plant Journal. 1998 ; Vol. 15, No. 3. pp. 301-310.
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