The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients

Kei Nanba, Shinichi Toyooka, Junichi Sou, Kazunori Tsukuda, Hiromasa Yamamoto, Akiko Sakai, Mamoru Oouchida, Naruyuki Kobayashi, Keitaro Matsuo, Norio Koide, Katsuyasu Kusaka, Kenji Shimizu, Hiroshi Date

Research output: Contribution to journalArticle

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Abstract

It has been reported that the rs1862214 single nucleotide polymorphism (SNP) in the programmed cell death 5 gene (PDCD5) is associated with smoking-related lung cancer risk and prognosis in a European population with a history of smoking. The aim of this study was to investigate the status and impact of SNPs in the PDCD5 locus of a Japanese population. We developed an assay based on real-time PCR with melting curve analysis for determining the rs1862214 SNP, and examined this SNP in 165 lung cancer patients and 180 healthy volunteers. Of the 165 lung cancer patients (107 smokers), 25 (17), 72 (47) and 68 (43) had the CC, CG and GG genotypes of rs1862214, respectively. Of the 180 volunteers (117 smokers), 31 (24), 81 (52) and 68 (41) had the CC, CG and GG genotypes of rs1862214, respectively. No significant difference in allelic distribution was found between Japanese patients and healthy controls, even among smokers. Based on the published data, the distribution of this SNP appears to be significantly different in Japanese and European populations. No significant difference in prognosis according to the SNP was observed, either in patients with a history of smoking or in the total number of patients. This too differs from the results from a European population. In conclusion, we developed a convenient real-time PCR-based assay for the genotyping of rs1862214 in the PDCD5 locus. The distribution of the rs1862214 SNP in our Japanese population differs from its distribution in a European population, and is not related to the risk of cancer or to poor prognosis in lung cancer. This suggests the presence of an ethnicity-related difference in the role of PDCD5 in the pathogenesis of lung cancer.

Original languageEnglish
Pages (from-to)667-671
Number of pages5
JournalMolecular Medicine Reports
Volume1
Issue number5
DOIs
Publication statusPublished - 2008

Fingerprint

Cell death
Polymorphism
Non-Small Cell Lung Carcinoma
Single Nucleotide Polymorphism
Cell Death
Nucleotides
Genes
Cells
Lung Neoplasms
Population
Smoking
Assays
Real-Time Polymerase Chain Reaction
Genotype
Melting
Freezing
Volunteers
Healthy Volunteers
Neoplasms

Keywords

  • Cancer risk
  • Japanese
  • Non-small cell lung cancer
  • PDCD5
  • Single nucleotide polymorphisms

ASJC Scopus subject areas

  • Biochemistry
  • Cancer Research
  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Oncology

Cite this

The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients. / Nanba, Kei; Toyooka, Shinichi; Sou, Junichi; Tsukuda, Kazunori; Yamamoto, Hiromasa; Sakai, Akiko; Oouchida, Mamoru; Kobayashi, Naruyuki; Matsuo, Keitaro; Koide, Norio; Kusaka, Katsuyasu; Shimizu, Kenji; Date, Hiroshi.

In: Molecular Medicine Reports, Vol. 1, No. 5, 2008, p. 667-671.

Research output: Contribution to journalArticle

Nanba, Kei ; Toyooka, Shinichi ; Sou, Junichi ; Tsukuda, Kazunori ; Yamamoto, Hiromasa ; Sakai, Akiko ; Oouchida, Mamoru ; Kobayashi, Naruyuki ; Matsuo, Keitaro ; Koide, Norio ; Kusaka, Katsuyasu ; Shimizu, Kenji ; Date, Hiroshi. / The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients. In: Molecular Medicine Reports. 2008 ; Vol. 1, No. 5. pp. 667-671.
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T1 - The allelic distribution of a single nucleotide polymorphism in the PDCD5 gene locus of Japanese non-small cell lung cancer patients

AU - Nanba, Kei

AU - Toyooka, Shinichi

AU - Sou, Junichi

AU - Tsukuda, Kazunori

AU - Yamamoto, Hiromasa

AU - Sakai, Akiko

AU - Oouchida, Mamoru

AU - Kobayashi, Naruyuki

AU - Matsuo, Keitaro

AU - Koide, Norio

AU - Kusaka, Katsuyasu

AU - Shimizu, Kenji

AU - Date, Hiroshi

PY - 2008

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