The advent of RNA interference in Entomology

Taro Mito, Taro Nakamura, Tetsuya Bando, Hideyo Ohuchi, Sumihare Noji

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

RNA interference (RNAi) is a cellular process by which an mRNA is targeted for degradation by a small interfering RNA that contains a strand complementary to a fragment of the target mRNA, resulting in sequence specific inhibition of gene expression. The discovery of RNAi enabled the use of loss-of-function analyses in many non-model insects other than Drosophila to elucidate the roles of specific genes. The RNAi approach has been widely used on insects in several fields, including embryogenesis, pattern formation, reproduction, biosynthesis and behavior. The increasing availability of insect genomes has made the RNAi technique an indispensable technique for characterizing gene functions in insects. Here we review the current status of RNAi-based experiments in insects and the applications of RNAi for species-specific insecticides, focusing on non-drosophilid insects. We also identify future applications for RNAi-based studies in Entomology.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalEntomological Science
Volume14
Issue number1
DOIs
Publication statusPublished - Jan 2011
Externally publishedYes

Fingerprint

entomology
RNA interference
RNA
insect
insects
small interfering RNA
gene
Drosophila
insecticides
gene expression
embryogenesis
genes
insecticide
biosynthesis
genome
degradation
methodology

Keywords

  • Development
  • Insecticides
  • Insects
  • Loss-of-function
  • Regeneration
  • RNAi

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Insect Science

Cite this

The advent of RNA interference in Entomology. / Mito, Taro; Nakamura, Taro; Bando, Tetsuya; Ohuchi, Hideyo; Noji, Sumihare.

In: Entomological Science, Vol. 14, No. 1, 01.2011, p. 1-8.

Research output: Contribution to journalArticle

Mito, Taro ; Nakamura, Taro ; Bando, Tetsuya ; Ohuchi, Hideyo ; Noji, Sumihare. / The advent of RNA interference in Entomology. In: Entomological Science. 2011 ; Vol. 14, No. 1. pp. 1-8.
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