Abstract
Brain-specific link protein Bral2 represents a substantial component of perineuronal nets (PNNs) enwrapping neurons in the central nervous system. To elucidate the role of Bral2 in auditory signal processing, the hearing function in knockout Bral2-/- (KO) mice was investigated using behavioral and electrophysiological methods and compared with wild type Bral2+/+ (WT) mice. The amplitudes of the acoustic startle reflex (ASR) and the efficiency of the prepulse inhibition of ASR (PPI of ASR), produced by prepulse noise stimulus or gap in continuous noise, was similar in 2-week-old WT and KO mice. Over the 2-month postnatal period the increase of ASR amplitudes was significantly more evident in WT mice than in KO mice. The efficiency of the PPI of ASR significantly increased in the 2-month postnatal period in WT mice, whereas in KO mice the PPI efficiency did not change. Hearing thresholds in 2-month-old WT mice, based on the auditory brainstem response (ABR) recordings, were significantly lower at high frequencies than in KO mice. However, amplitudes and peak latencies of individual waves of click-evoked ABR did not differ significantly between WT and KO mice. Temporal resolution and neural adaptation were significantly better in 2-month-old WT mice than in age-matched KO mice. These results support a hypothesis that the absence of perineuronal net formation at the end of the developmental period in the KO mice results in higher hearing threshold at high frequencies and weaker temporal resolution ability in adult KO animals compared to WT mice.
| Original language | English |
|---|---|
| Pages (from-to) | 867-880 |
| Number of pages | 14 |
| Journal | Physiological Research |
| Volume | 66 |
| Issue number | 5 |
| Publication status | Published - Jan 1 2017 |
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Keywords
- Acoustic startle reflex
- Auditory brainstem response
- Critical period
- Perineuronal nets
- Prepulse inhibition
ASJC Scopus subject areas
- Physiology
Cite this
The absence of brain-specific link protein Bral2 in perineuronal nets hampers auditory temporal resolution and neural adaptation in mice. / Popelář, J.; Díaz Gómez, M.; Lindovskỳ, J.; Rybalko, N.; Burianová, J.; Oohashi, Toshitaka; Syka, J.
In: Physiological Research, Vol. 66, No. 5, 01.01.2017, p. 867-880.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The absence of brain-specific link protein Bral2 in perineuronal nets hampers auditory temporal resolution and neural adaptation in mice
AU - Popelář, J.
AU - Díaz Gómez, M.
AU - Lindovskỳ, J.
AU - Rybalko, N.
AU - Burianová, J.
AU - Oohashi, Toshitaka
AU - Syka, J.
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Brain-specific link protein Bral2 represents a substantial component of perineuronal nets (PNNs) enwrapping neurons in the central nervous system. To elucidate the role of Bral2 in auditory signal processing, the hearing function in knockout Bral2-/- (KO) mice was investigated using behavioral and electrophysiological methods and compared with wild type Bral2+/+ (WT) mice. The amplitudes of the acoustic startle reflex (ASR) and the efficiency of the prepulse inhibition of ASR (PPI of ASR), produced by prepulse noise stimulus or gap in continuous noise, was similar in 2-week-old WT and KO mice. Over the 2-month postnatal period the increase of ASR amplitudes was significantly more evident in WT mice than in KO mice. The efficiency of the PPI of ASR significantly increased in the 2-month postnatal period in WT mice, whereas in KO mice the PPI efficiency did not change. Hearing thresholds in 2-month-old WT mice, based on the auditory brainstem response (ABR) recordings, were significantly lower at high frequencies than in KO mice. However, amplitudes and peak latencies of individual waves of click-evoked ABR did not differ significantly between WT and KO mice. Temporal resolution and neural adaptation were significantly better in 2-month-old WT mice than in age-matched KO mice. These results support a hypothesis that the absence of perineuronal net formation at the end of the developmental period in the KO mice results in higher hearing threshold at high frequencies and weaker temporal resolution ability in adult KO animals compared to WT mice.
AB - Brain-specific link protein Bral2 represents a substantial component of perineuronal nets (PNNs) enwrapping neurons in the central nervous system. To elucidate the role of Bral2 in auditory signal processing, the hearing function in knockout Bral2-/- (KO) mice was investigated using behavioral and electrophysiological methods and compared with wild type Bral2+/+ (WT) mice. The amplitudes of the acoustic startle reflex (ASR) and the efficiency of the prepulse inhibition of ASR (PPI of ASR), produced by prepulse noise stimulus or gap in continuous noise, was similar in 2-week-old WT and KO mice. Over the 2-month postnatal period the increase of ASR amplitudes was significantly more evident in WT mice than in KO mice. The efficiency of the PPI of ASR significantly increased in the 2-month postnatal period in WT mice, whereas in KO mice the PPI efficiency did not change. Hearing thresholds in 2-month-old WT mice, based on the auditory brainstem response (ABR) recordings, were significantly lower at high frequencies than in KO mice. However, amplitudes and peak latencies of individual waves of click-evoked ABR did not differ significantly between WT and KO mice. Temporal resolution and neural adaptation were significantly better in 2-month-old WT mice than in age-matched KO mice. These results support a hypothesis that the absence of perineuronal net formation at the end of the developmental period in the KO mice results in higher hearing threshold at high frequencies and weaker temporal resolution ability in adult KO animals compared to WT mice.
KW - Acoustic startle reflex
KW - Auditory brainstem response
KW - Critical period
KW - Perineuronal nets
KW - Prepulse inhibition
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UR - http://www.scopus.com/inward/citedby.url?scp=85030692894&partnerID=8YFLogxK
M3 - Article
VL - 66
SP - 867
EP - 880
JO - Physiological Research
JF - Physiological Research
SN - 0862-8408
IS - 5
ER -