The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source

Shin-ichi Miyoshi, Takehito Kamei, Yu Inami, Yuki Ota, Shigeo Yamamoto, Ken Ichi Tomochika, Sumio Shinoda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Vibrio vulnificus, an opportunistic human pathogen, can obtain iron from a variety of heme proteins. This process involves the digestion of heme proteins by an exoprotease to liberate protoheme (iron-protoporphyrin IX). In the present study, we tested whether this pathogen also uses a synthetic heme compound, Fe-α,β,γ,δ-tetraphenylporphine tetrasulfonic acid (Fe-TPPS), as an iron source. When inoculated into a medium containing Fe-TPPS, V. vulnificus L-180 multiplication was seen to be dependent on the concentration of the synthetic heme compound; a mutant lacking the ability to utilize protoheme did not multiply. Cells of the strain grown under the iron-restricted condition showed time-dependent uptake of Fe-TPPS. The ability to use either protoheme or Fe-TPPS was significantly reduced by the addition of an excess amount of free TPPS or Cu-TPPS. The data suggest that, V. vulnificus may assimilate Fe-TPPS, at least partially, through the same system as that for protoheme. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish
Pages (from-to)73-77
Number of pages5
JournalFEMS Microbiology Letters
Volume172
Issue number1
DOIs
Publication statusPublished - Mar 1 1999

Fingerprint

Vibrio vulnificus
Heme
Iron
Acids
Hemeproteins
Exopeptidases
tetraphenylporphine
Digestion

Keywords

  • Fe-TPPS
  • Heme utilization
  • Vibrio vulnificus

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source. / Miyoshi, Shin-ichi; Kamei, Takehito; Inami, Yu; Ota, Yuki; Yamamoto, Shigeo; Tomochika, Ken Ichi; Shinoda, Sumio.

In: FEMS Microbiology Letters, Vol. 172, No. 1, 01.03.1999, p. 73-77.

Research output: Contribution to journalArticle

Miyoshi, Shin-ichi ; Kamei, Takehito ; Inami, Yu ; Ota, Yuki ; Yamamoto, Shigeo ; Tomochika, Ken Ichi ; Shinoda, Sumio. / The ability of Vibrio vulnificus to use a synthetic hydrophilic heme compound, Fe-TPPS, as a single iron source. In: FEMS Microbiology Letters. 1999 ; Vol. 172, No. 1. pp. 73-77.
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