Abstract
Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.
| Original language | English |
|---|---|
| Pages (from-to) | 433-438 |
| Number of pages | 6 |
| Journal | Journal of Hard Tissue Biology |
| Volume | 22 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2013 |
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Keywords
- Bone marrow-derived cells
- Differentiation
- Green fluorescent protein
- Parenchymal cells
- Salivary gland
ASJC Scopus subject areas
- Biochemistry
- Cell Biology
- Biomaterials
- Medicine (miscellaneous)
- Orthopedics and Sports Medicine
- Dentistry(all)
Cite this
The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands. / Yuan, Yao Wei; Tamamura, Ryo; Lei, Lei; Katase, Naoki; Sathi, Gulsan Ara; Ito, Satoshi; Tsujigiwa, Hidetsugu; Nagatsuka, Hitoshi.
In: Journal of Hard Tissue Biology, Vol. 22, No. 4, 2013, p. 433-438.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands
AU - Yuan, Yao Wei
AU - Tamamura, Ryo
AU - Lei, Lei
AU - Katase, Naoki
AU - Sathi, Gulsan Ara
AU - Ito, Satoshi
AU - Tsujigiwa, Hidetsugu
AU - Nagatsuka, Hitoshi
PY - 2013
Y1 - 2013
N2 - Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.
AB - Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.
KW - Bone marrow-derived cells
KW - Differentiation
KW - Green fluorescent protein
KW - Parenchymal cells
KW - Salivary gland
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UR - http://www.scopus.com/inward/citedby.url?scp=84887076699&partnerID=8YFLogxK
U2 - 10.2485/jhtb.22.433
DO - 10.2485/jhtb.22.433
M3 - Article
VL - 22
SP - 433
EP - 438
JO - Journal of Hard Tissue Biology
JF - Journal of Hard Tissue Biology
SN - 1341-7649
IS - 4
ER -