The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands

Yao Wei Yuan, Ryo Tamamura, Lei Lei, Naoki Katase, Gulsan Ara Sathi, Satoshi Ito, Hidetsugu Tsujigiwa, Hitoshi Nagatsuka

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.

Original languageEnglish
Pages (from-to)433-438
Number of pages6
JournalJournal of Hard Tissue Biology
Volume22
Issue number4
DOIs
Publication statusPublished - 2013

Fingerprint

Salivary Glands
Bone Marrow Cells
Bone
Green Fluorescent Proteins
Cell engineering
Proteins
Amylases
Exocrine Glands
Cell Engineering
Stem cells
Acinar Cells
Tissue engineering
Submandibular Gland
Parotid Gland
Oral Health
Tissue Engineering
Plasticity
Cell- and Tissue-Based Therapy
Inbred C57BL Mouse
Cells

Keywords

  • Bone marrow-derived cells
  • Differentiation
  • Green fluorescent protein
  • Parenchymal cells
  • Salivary gland

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biomaterials
  • Medicine (miscellaneous)
  • Orthopedics and Sports Medicine
  • Dentistry(all)

Cite this

The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands. / Yuan, Yao Wei; Tamamura, Ryo; Lei, Lei; Katase, Naoki; Sathi, Gulsan Ara; Ito, Satoshi; Tsujigiwa, Hidetsugu; Nagatsuka, Hitoshi.

In: Journal of Hard Tissue Biology, Vol. 22, No. 4, 2013, p. 433-438.

Research output: Contribution to journalArticle

Yuan, Yao Wei ; Tamamura, Ryo ; Lei, Lei ; Katase, Naoki ; Sathi, Gulsan Ara ; Ito, Satoshi ; Tsujigiwa, Hidetsugu ; Nagatsuka, Hitoshi. / The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands. In: Journal of Hard Tissue Biology. 2013 ; Vol. 22, No. 4. pp. 433-438.
@article{84abd079ca6244af8d9646ae3a1d1442,
title = "The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands",
abstract = "Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.",
keywords = "Bone marrow-derived cells, Differentiation, Green fluorescent protein, Parenchymal cells, Salivary gland",
author = "Yuan, {Yao Wei} and Ryo Tamamura and Lei Lei and Naoki Katase and Sathi, {Gulsan Ara} and Satoshi Ito and Hidetsugu Tsujigiwa and Hitoshi Nagatsuka",
year = "2013",
doi = "10.2485/jhtb.22.433",
language = "English",
volume = "22",
pages = "433--438",
journal = "Journal of Hard Tissue Biology",
issn = "1341-7649",
publisher = "Society of Hard Tissue Regenerative Biology",
number = "4",

}

TY - JOUR

T1 - The ability of transplanted bone marrow-derived cells to differentiate into parenchymal cells of salivary glands

AU - Yuan, Yao Wei

AU - Tamamura, Ryo

AU - Lei, Lei

AU - Katase, Naoki

AU - Sathi, Gulsan Ara

AU - Ito, Satoshi

AU - Tsujigiwa, Hidetsugu

AU - Nagatsuka, Hitoshi

PY - 2013

Y1 - 2013

N2 - Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.

AB - Salivary glands are important exocrine glands for oral health and initial digestion. Salivary gland dysfunction resulting from irreversible glandular damage usually leads to poor life quality in patients. Recent investigations showed that bone marrow-derived cells (BMDCs) could be grafted into non-hematopoietic cells in multiple tissues. In this study, BMDCs from green fluorescent protein (GFP) mice were transplanted into irradiated C57BL/6 mice to assess the ability of BMDCs to differentiate into parenchymal cells of salivary glands by immunohistochemistry and double fluorescent staining. The data revealed that a population of GFP positive cells showed the characteristics of acinar cells, ductal cells and myoepithelial cells in parotid and submandibular glands, and that some of BMDCs presented amylase expression. These results showed that BMDCs have the ability to differentiate into parenchymal cells of salivary glands with certain glandular cell functions. Such plasticity of BMDCs can be the first step for salivary gland regeneration by stem cells and tissue engineering therapy.

KW - Bone marrow-derived cells

KW - Differentiation

KW - Green fluorescent protein

KW - Parenchymal cells

KW - Salivary gland

UR - http://www.scopus.com/inward/record.url?scp=84887076699&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84887076699&partnerID=8YFLogxK

U2 - 10.2485/jhtb.22.433

DO - 10.2485/jhtb.22.433

M3 - Article

VL - 22

SP - 433

EP - 438

JO - Journal of Hard Tissue Biology

JF - Journal of Hard Tissue Biology

SN - 1341-7649

IS - 4

ER -