The ABC transporter ABCG36 is required for cadmium tolerance in rice

Shan Fu, Youshe Lu, Xiang Zhang, Guangzhe Yang, Dong Chao, Zhigang Wang, Mingxing Shi, Jiugeng Chen, Dai Yin Chao, Rongbai Li, Jian Feng Ma, Jixing Xia, Hendrik Küpper

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cadmium (Cd) is a highly toxic heavy metal in nature, which causes severe damage to plant growth. The molecular mechanisms for Cd detoxification are poorly understood. Here, we report that a G-type ATP-binding cassette transporter, OsABCG36, is involved in Cd tolerance in rice. OsABCG36 was expressed in both roots and shoots at a low level, but expression in the roots rather than the shoots was greatly up-regulated by a short exposure to Cd. A spatial expression analysis showed that Cd-induced expression of OsABCG36 was found in both the root tip and the mature root region. Transient expression of OsABCG36 in rice protoplast cells showed that it was localized to the plasma membrane. Immunostaining showed that OsABCG36 was localized in all root cells except the epidermal cells. Knockout of OsABCG36 resulted in increased Cd accumulation in root cell sap and enhanced Cd sensitivity, but did not affect tolerance to other metals including Al, Zn, Cu, and Pb. The concentration of Cd in the shoots was similar between the knockout lines and wild-type rice. Heterologous expression of OsABCG36 in yeast showed an efflux activity for Cd, but not for Zn. Taken together, our results indicate that OsABCG36 is not involved in Cd accumulation in the shoots, but is required for Cd tolerance by exporting Cd or Cd conjugates from the root cells in rice.

Original languageEnglish
Pages (from-to)5909-5918
Number of pages10
JournalJournal of experimental botany
Volume70
Issue number20
DOIs
Publication statusPublished - Oct 24 2019

Fingerprint

ABC transporters
ATP-Binding Cassette Transporters
Cadmium
cadmium
rice
shoots
Oryza
cells
Spatial Analysis
Meristem
Protoplasts
Poisons
Heavy Metals
root tips
sap
protoplasts
heavy metals
plasma membrane

Keywords

  • ABC transporter
  • admium
  • Cd accumulation
  • Cd tolerance
  • ice
  • OsABCG36

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Fu, S., Lu, Y., Zhang, X., Yang, G., Chao, D., Wang, Z., ... Küpper, H. (2019). The ABC transporter ABCG36 is required for cadmium tolerance in rice. Journal of experimental botany, 70(20), 5909-5918. https://doi.org/10.1093/jxb/erz335

The ABC transporter ABCG36 is required for cadmium tolerance in rice. / Fu, Shan; Lu, Youshe; Zhang, Xiang; Yang, Guangzhe; Chao, Dong; Wang, Zhigang; Shi, Mingxing; Chen, Jiugeng; Chao, Dai Yin; Li, Rongbai; Ma, Jian Feng; Xia, Jixing; Küpper, Hendrik.

In: Journal of experimental botany, Vol. 70, No. 20, 24.10.2019, p. 5909-5918.

Research output: Contribution to journalArticle

Fu, S, Lu, Y, Zhang, X, Yang, G, Chao, D, Wang, Z, Shi, M, Chen, J, Chao, DY, Li, R, Ma, JF, Xia, J & Küpper, H 2019, 'The ABC transporter ABCG36 is required for cadmium tolerance in rice', Journal of experimental botany, vol. 70, no. 20, pp. 5909-5918. https://doi.org/10.1093/jxb/erz335
Fu, Shan ; Lu, Youshe ; Zhang, Xiang ; Yang, Guangzhe ; Chao, Dong ; Wang, Zhigang ; Shi, Mingxing ; Chen, Jiugeng ; Chao, Dai Yin ; Li, Rongbai ; Ma, Jian Feng ; Xia, Jixing ; Küpper, Hendrik. / The ABC transporter ABCG36 is required for cadmium tolerance in rice. In: Journal of experimental botany. 2019 ; Vol. 70, No. 20. pp. 5909-5918.
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