The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer

Ikuo Sekine, Mitsuo Sato, Noriaki Sunaga, Shinichi Toyooka, Michael Peyton, Ramon Parsons, Weidong Wang, Adi F. Gazdar, John D. Minna

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

BAF180 encoding a subunit of the human SWI/SNF chromatin remodeling complex maps to 3p21, in a region where frequent allele loss has been detected in lung cancer. BAF180 can be mutated and has tumor suppressing properties in breast cancer. In addition, another member of this complex, hSNF5/INI1, is a known tumor suppressor gene (TSG) for malignant rhabdoid and childhood central nervous system tumors. Thus, BAF180 is a strong candidate TSG for lung cancer. The objective of this study was to determine whether BAF180 mRNA or protein expression was inactivated or abnormal in lung cancers to prompt detailed DNA promoter methylation or mutational analyses. In 30 non-small-cell and 26 small-cell lung cancer cell lines, most of which had 3p21 allele loss, BAF180 mRNA and protein expression were evaluated by RT-PCR using three sets of primers and Western blotting using two anti-BAF180 antibodies. In all cases, BAF180 was expressed and no abnormal size BAF180 protein was detected. Finally, we found no amino-acid sequence coding mutations in five non-small-cell and five small-cell lung cancer cell lines, while we did find a new splicing isoform of BAF180 (AY281068). We conclude that abnormalities of BAF180 are not frequently involved in the pathogenesis of lung cancer.

Original languageEnglish
Pages (from-to)2735-2738
Number of pages4
JournalOncogene
Volume24
Issue number16
DOIs
Publication statusPublished - Apr 14 2005
Externally publishedYes

Fingerprint

Tumor Suppressor Genes
Lung Neoplasms
Small Cell Lung Carcinoma
Alleles
Cell Line
Central Nervous System Neoplasms
Messenger RNA
Proteins
Chromatin Assembly and Disassembly
DNA Methylation
Anti-Idiotypic Antibodies
Amino Acid Sequence
Protein Isoforms
Western Blotting
Breast Neoplasms
Polymerase Chain Reaction
Mutation
Neoplasms

Keywords

  • BAF180
  • Chromatin remodeling factor
  • Lung cancer
  • Tumor suppressor gene

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Sekine, I., Sato, M., Sunaga, N., Toyooka, S., Peyton, M., Parsons, R., ... Minna, J. D. (2005). The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer. Oncogene, 24(16), 2735-2738. https://doi.org/10.1038/sj.onc.1207694

The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer. / Sekine, Ikuo; Sato, Mitsuo; Sunaga, Noriaki; Toyooka, Shinichi; Peyton, Michael; Parsons, Ramon; Wang, Weidong; Gazdar, Adi F.; Minna, John D.

In: Oncogene, Vol. 24, No. 16, 14.04.2005, p. 2735-2738.

Research output: Contribution to journalArticle

Sekine, I, Sato, M, Sunaga, N, Toyooka, S, Peyton, M, Parsons, R, Wang, W, Gazdar, AF & Minna, JD 2005, 'The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer', Oncogene, vol. 24, no. 16, pp. 2735-2738. https://doi.org/10.1038/sj.onc.1207694
Sekine, Ikuo ; Sato, Mitsuo ; Sunaga, Noriaki ; Toyooka, Shinichi ; Peyton, Michael ; Parsons, Ramon ; Wang, Weidong ; Gazdar, Adi F. ; Minna, John D. / The 3p21 candidate tumor suppressor gene BAF180 is normally expressed in human lung cancer. In: Oncogene. 2005 ; Vol. 24, No. 16. pp. 2735-2738.
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