The 3'-untranslated region of ADAMTS1 regulates its mRNA stability

Omer Faruk Hatipoglu, Satoshi Hirohata, Kursat Oguz Yaykasli, Mehmet Zeynel Cilek, Kadir Demircan, Ryoko Shinohata, Tomoko Yonezawa, Toshitaka Oohashi, Shozo Kusachi, Yoshifumi Ninomiya

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

ADAMTS1 (a disintegrin and metalloproteinase with thrombospondin motifs 1) is an inflammatoryinduced gene. We have previously reported that ADAMTS1 was strongly but transiently expressed in the infarcted heart. In this study, we investigated whether a 3'-untranslated region (UTR) affects the mRNA stability of this gene. When stimulated with tissue necrosis factor (TNF)-alpha, the expression level of ADAMTS1 mRNA rapidly increased, but the induction of ADAMTS1 mRNA peaked at 6h after stimulation, after which the expression levels of ADAMTS1 mRNA decreased. The 3'-UTR ADAMTS1 mRNA contains multiple adenine and uridine-rich elements, suggesting that the 3'-UTR may regulate gene stability. The addition of actinomycin D, an RNA synthesis inhibitor, demonstrated the decay of induced ADAMTS1 mRNA by TNF-alpha. Furthermore, a region containing multiple AUUUA motifs within the ADAMTS1 3'-UTR destabilized transfected Enhanced Green Fluorescence Protein (EGFP) mRNA expression. These results demonstrated that the ADAMTS1 3'-UTR may regulate the expression of ADAMTS1 mRNA.

Original languageEnglish
Pages (from-to)79-85
Number of pages7
JournalActa medica Okayama
Volume63
Issue number2
Publication statusPublished - May 2009

Keywords

  • ADAMTS1
  • Gene regulation
  • Metalloproteinase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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  • Cite this

    Hatipoglu, O. F., Hirohata, S., Yaykasli, K. O., Cilek, M. Z., Demircan, K., Shinohata, R., Yonezawa, T., Oohashi, T., Kusachi, S., & Ninomiya, Y. (2009). The 3'-untranslated region of ADAMTS1 regulates its mRNA stability. Acta medica Okayama, 63(2), 79-85.