Abstract
To investigate the role of flagella and monomer flagellin in the interaction between Pseudomonas syringae pv. tabaci and plants, non-polar fliC and fliD mutants were produced. The ORFs for fliC and fliD are deleted in the ΔfliC and ΔfliD mutants, respectively. Both mutants lost all flagella and were non-motile. The ΔfliC mutant did not produce flagellin, whereas the ΔfliD mutant, which lacks the HAP2 protein, secreted large amounts of monomer flagellin into the culture medium. Inoculation of non-host tomato leaves with wild-type P. syringae pv. tabaci or the ΔfliD mutant induced a hypersensitive reaction (HR), whereas the ΔfliC mutant propagated and caused characteristic symptom-like changes. In tomato cells in suspension culture, wild-type P. syringae pv. tabaci induced slight, visible HR-like changes. The ΔfliC mutant did not induce HR, but the ΔfliD mutant induced a remarkably strong HR. Expression of the hsr203J gene was rapidly and strongly induced by inoculation with the ΔfliD mutant, compared to inoculation with wild-type P. syringae pv. tabaci. Furthermore, introduction of the fliC gene into the ΔfliC mutant restored motility and HR-inducing ability in tomato. These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells.
| Original language | English |
|---|---|
| Pages (from-to) | 21-30 |
| Number of pages | 10 |
| Journal | Molecular Genetics and Genomics |
| Volume | 269 |
| Issue number | 1 |
| Publication status | Published - Apr 1 2003 |
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Keywords
- Elicitor
- Flagella
- Flagellin
- fliCD
- Hypersensitive reaction (HR)
ASJC Scopus subject areas
- Genetics
Cite this
The ΔfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells. / Shimizu, R.; Taguchi, F.; Marutani, M.; Mukaihara, T.; Inagaki, Y.; Toyoda, Kazuhiro; Shiraishi, T.; Ichinose, Yuki.
In: Molecular Genetics and Genomics, Vol. 269, No. 1, 01.04.2003, p. 21-30.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The ΔfliD mutant of Pseudomonas syringae pv. tabaci, which secretes flagellin monomers, induces a strong hypersensitive reaction (HR) in non-host tomato cells
AU - Shimizu, R.
AU - Taguchi, F.
AU - Marutani, M.
AU - Mukaihara, T.
AU - Inagaki, Y.
AU - Toyoda, Kazuhiro
AU - Shiraishi, T.
AU - Ichinose, Yuki
PY - 2003/4/1
Y1 - 2003/4/1
N2 - To investigate the role of flagella and monomer flagellin in the interaction between Pseudomonas syringae pv. tabaci and plants, non-polar fliC and fliD mutants were produced. The ORFs for fliC and fliD are deleted in the ΔfliC and ΔfliD mutants, respectively. Both mutants lost all flagella and were non-motile. The ΔfliC mutant did not produce flagellin, whereas the ΔfliD mutant, which lacks the HAP2 protein, secreted large amounts of monomer flagellin into the culture medium. Inoculation of non-host tomato leaves with wild-type P. syringae pv. tabaci or the ΔfliD mutant induced a hypersensitive reaction (HR), whereas the ΔfliC mutant propagated and caused characteristic symptom-like changes. In tomato cells in suspension culture, wild-type P. syringae pv. tabaci induced slight, visible HR-like changes. The ΔfliC mutant did not induce HR, but the ΔfliD mutant induced a remarkably strong HR. Expression of the hsr203J gene was rapidly and strongly induced by inoculation with the ΔfliD mutant, compared to inoculation with wild-type P. syringae pv. tabaci. Furthermore, introduction of the fliC gene into the ΔfliC mutant restored motility and HR-inducing ability in tomato. These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells.
AB - To investigate the role of flagella and monomer flagellin in the interaction between Pseudomonas syringae pv. tabaci and plants, non-polar fliC and fliD mutants were produced. The ORFs for fliC and fliD are deleted in the ΔfliC and ΔfliD mutants, respectively. Both mutants lost all flagella and were non-motile. The ΔfliC mutant did not produce flagellin, whereas the ΔfliD mutant, which lacks the HAP2 protein, secreted large amounts of monomer flagellin into the culture medium. Inoculation of non-host tomato leaves with wild-type P. syringae pv. tabaci or the ΔfliD mutant induced a hypersensitive reaction (HR), whereas the ΔfliC mutant propagated and caused characteristic symptom-like changes. In tomato cells in suspension culture, wild-type P. syringae pv. tabaci induced slight, visible HR-like changes. The ΔfliC mutant did not induce HR, but the ΔfliD mutant induced a remarkably strong HR. Expression of the hsr203J gene was rapidly and strongly induced by inoculation with the ΔfliD mutant, compared to inoculation with wild-type P. syringae pv. tabaci. Furthermore, introduction of the fliC gene into the ΔfliC mutant restored motility and HR-inducing ability in tomato. These results, together with our previous study, suggest that the flagellin monomer of pv. tabaci acts as a strong elicitor to induce HR-associated cell death in non-host tomato cells.
KW - Elicitor
KW - Flagella
KW - Flagellin
KW - fliCD
KW - Hypersensitive reaction (HR)
UR - http://www.scopus.com/inward/record.url?scp=0013147211&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0013147211&partnerID=8YFLogxK
M3 - Article
C2 - 12715150
AN - SCOPUS:0013147211
VL - 269
SP - 21
EP - 30
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 1
ER -