TGF-β induces the differentiation of bone marrow stem cells into immature cardiomyocytes

Tao Sheng Li, Takahiro Komota, Mako Ohshima, Shu Lan Qin, Masayuki Kubo, Kazuhiro Ueda, Kimikazu Hamano

Research output: Contribution to journalArticle

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Abstract

Transforming growth factor-β1 (TGF-β1) was found to induce the myogenic differentiation of bone marrow stem cells (BMSC). We investigated the morphological and electrophysiological properties of differentiated cells, and the mechanisms related to TGF-β1-induced myogenic differentiation. We found that TGF-β increased the expression of the cardiac transcription factors, GATA-4 and NKx-2.5, in BMSC after 1-3 days of cultivation. TGF-β also induced the expressions of cardiac myosin, troponins, and ANP after 3-14 days of cultivation. However, the Ca2+ transient was relatively weak, the connexin-43 expression was irregular, and spontaneous beating was not detected within 28 days observation. TGF-β stimulation up-regulated most of the TGF-β BMP signaling pathway genes, including TGFBI, ACVR2B, and phosphorylated Smad-2 and Smad-3, within 24 h. TGF-β induced the differentiation of BMSC into immature cardiomyocytes by activating the TGF-β BMP signaling pathway.

Original languageEnglish
Pages (from-to)1074-1080
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number4
DOIs
Publication statusPublished - Feb 22 2008
Externally publishedYes

Fingerprint

Stem cells
Cardiac Myocytes
Bone Marrow Cells
Bone
Stem Cells
Transforming Growth Factors
GATA4 Transcription Factor
Cardiac Myosins
Connexin 43
Troponin
Atrial Natriuretic Factor
Genes
Observation

Keywords

  • Differentiation
  • Stem cell
  • TGF-β

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

TGF-β induces the differentiation of bone marrow stem cells into immature cardiomyocytes. / Li, Tao Sheng; Komota, Takahiro; Ohshima, Mako; Qin, Shu Lan; Kubo, Masayuki; Ueda, Kazuhiro; Hamano, Kimikazu.

In: Biochemical and Biophysical Research Communications, Vol. 366, No. 4, 22.02.2008, p. 1074-1080.

Research output: Contribution to journalArticle

Li, Tao Sheng ; Komota, Takahiro ; Ohshima, Mako ; Qin, Shu Lan ; Kubo, Masayuki ; Ueda, Kazuhiro ; Hamano, Kimikazu. / TGF-β induces the differentiation of bone marrow stem cells into immature cardiomyocytes. In: Biochemical and Biophysical Research Communications. 2008 ; Vol. 366, No. 4. pp. 1074-1080.
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