TGF-β induces the differentiation of bone marrow stem cells into immature cardiomyocytes

Tao Sheng Li, Takahiro Komota, Mako Ohshima, Shu Lan Qin, Masayuki Kubo, Kazuhiro Ueda, Kimikazu Hamano

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45 Citations (Scopus)

Abstract

Transforming growth factor-β1 (TGF-β1) was found to induce the myogenic differentiation of bone marrow stem cells (BMSC). We investigated the morphological and electrophysiological properties of differentiated cells, and the mechanisms related to TGF-β1-induced myogenic differentiation. We found that TGF-β increased the expression of the cardiac transcription factors, GATA-4 and NKx-2.5, in BMSC after 1-3 days of cultivation. TGF-β also induced the expressions of cardiac myosin, troponins, and ANP after 3-14 days of cultivation. However, the Ca2+ transient was relatively weak, the connexin-43 expression was irregular, and spontaneous beating was not detected within 28 days observation. TGF-β stimulation up-regulated most of the TGF-β BMP signaling pathway genes, including TGFBI, ACVR2B, and phosphorylated Smad-2 and Smad-3, within 24 h. TGF-β induced the differentiation of BMSC into immature cardiomyocytes by activating the TGF-β BMP signaling pathway.

Original languageEnglish
Pages (from-to)1074-1080
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume366
Issue number4
DOIs
Publication statusPublished - Feb 22 2008

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Keywords

  • Differentiation
  • Stem cell
  • TGF-β

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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