Tetrodotoxin-blockable depolarization-activated Na+ currents in a cultured endothelial cell line derived from rat interlobar arter and human umbilical vein

Dmitri V. Gordienko, Hirokazu Tsukahara

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

Voltage-dependent Na+ current (INa) was identified in cultured endothelial cells derived from rat interlobar artery (RIAE cells) and human umbilical vein (HUVE cells). Tetrodotoxin (TTX) reduced INa in a dose-dependent manner with the apparent dissociation constant (Kd) of 1.4 μM. Low sensitivity of INa to TTX as well as its kinetics and voltage-dependent properties indicates that voltage-gated Na channels expressed in vascular endothelial cells belong to the so-called TTX-resistant type [2].

Original languageEnglish
Pages (from-to)91-93
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume428
Issue number1
DOIs
Publication statusPublished - Aug 1 1994
Externally publishedYes

Keywords

  • Na channel
  • Patch-clamp technique
  • Tetrodotoxin
  • Vascular endothelium

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Physiology (medical)

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