Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

Hina Satone, Shohei Nonaka, Jae Man Lee, Yohei Shimasaki, Takahiro Kusakabe, Shun ichiro Kawabata, Yuji Oshima

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

Original languageEnglish
Pages (from-to)50-52
Number of pages3
JournalToxicon
Volume125
DOIs
Publication statusPublished - Jan 1 2017

Keywords

  • Binding assay
  • Pufferfish saxitoxin and tetrodotoxin binding proteins
  • Tetrodotoxin

ASJC Scopus subject areas

  • Toxicology

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