Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes

Hina Satone, Shohei Nonaka, Jae Man Lee, Yohei Shimasaki, Takahiro Kusakabe, Shun ichiro Kawabata, Yuji Oshima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We investigated the ability of recombinant pufferfish saxitoxin and tetrodotoxin binding protein types 1 and 2 of Takifugu rubripes (rTrub.PSTBP1 and rTrub.PSTBP2) to bind to tetrodotoxin (TTX) and tributyltin. Both rTrub.PSTBPs bound to tributyltin in an ultrafiltration binding assay but lost this ability on heat denaturation. In contrast, only rTrub.PSTBP2 bound to TTX even heat denaturation. This result suggests that the amino acid sequence of PSTBP2 may be contributed for its affinity for TTX.

Original languageEnglish
Pages (from-to)50-52
Number of pages3
JournalToxicon
Volume125
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Takifugu
Saxitoxin
Tetraodontiformes
Tetrodotoxin
Denaturation
Hot Temperature
Ultrafiltration
Amino Acid Sequence
Assays
Amino Acids
tributyltin
tetrodotoxin-binding protein

Keywords

  • Binding assay
  • Pufferfish saxitoxin and tetrodotoxin binding proteins
  • Tetrodotoxin

ASJC Scopus subject areas

  • Toxicology

Cite this

Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes. / Satone, Hina; Nonaka, Shohei; Lee, Jae Man; Shimasaki, Yohei; Kusakabe, Takahiro; Kawabata, Shun ichiro; Oshima, Yuji.

In: Toxicon, Vol. 125, 01.01.2017, p. 50-52.

Research output: Contribution to journalArticle

Satone, Hina ; Nonaka, Shohei ; Lee, Jae Man ; Shimasaki, Yohei ; Kusakabe, Takahiro ; Kawabata, Shun ichiro ; Oshima, Yuji. / Tetrodotoxin- and tributyltin-binding abilities of recombinant pufferfish saxitoxin and tetrodotoxin binding proteins of Takifugu rubripes. In: Toxicon. 2017 ; Vol. 125. pp. 50-52.
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AU - Kawabata, Shun ichiro

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