Tetrad-FISH analysis reveals recombination suppression by interstitial heterochromatin sequences in rye (Secale cereale)

N. Kagawa, Kiyotaka Nagaki, H. Tsujimoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Tetrad analysis is a genetic method that can locate genes and centromeres on a linkage map with a high degree of precision. Despite its effectiveness and accuracy, application of this method is generally limited to fungi, algae and mosses. Here we demonstrate a new method of tetrad analysis that is applicable to other organisms. This combines tetrad analysis with fluorescence in situ hybridization (FISH), and is thus referred to as tetrad-FISH analysis. We demonstrate the effectiveness of this method using tetrads of rye, Secale cereale. The rye strain JNK contains interstitial heterochromatin in a region of Chromosome 2R. We have previously cloned the tandemly repeated sequence forming this heterochromatin in the plasmid pScJNK. We performed FISH using pScJNK as the probe on tetrads obtained from heterozygotes for the heterochromatin region. The frequency of tetrads demonstrating positive signals in two cells that are diagonally opposite one another must correspond to the frequency of recombination in the interval between the heterochromatin and the centromere. Comparison between the results of tetrad-FISH analysis and linkage maps based on RFLP markers clearly indicated that heterochromatin strongly suppresses recombination of whole chromosomal regions. We discuss the effectiveness of tetrad-FISH analysis, particularly for the localization of functional centromeres in linkage maps.

Original languageEnglish
Pages (from-to)10-15
Number of pages6
JournalMolecular Genetics and Genomics
Volume267
Issue number1
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Heterochromatin
Fluorescence In Situ Hybridization
Genetic Recombination
Centromere
Bryophyta
Heterozygote
Restriction Fragment Length Polymorphisms
Plasmids
Fungi
Chromosomes
Secale
Genes

Keywords

  • Centromere
  • Heterochromatin
  • Recombination suppression
  • Secale cereale
  • Tetrad-FISH analysis

ASJC Scopus subject areas

  • Genetics

Cite this

Tetrad-FISH analysis reveals recombination suppression by interstitial heterochromatin sequences in rye (Secale cereale). / Kagawa, N.; Nagaki, Kiyotaka; Tsujimoto, H.

In: Molecular Genetics and Genomics, Vol. 267, No. 1, 2002, p. 10-15.

Research output: Contribution to journalArticle

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