Tethering assays for COPI vesicles mediated by golgins

Ayano Satoh, Jörg Malsam, Graham Warren

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A method is described that allows the attachment of COPI vesicles and Golgi membranes to glass slides that can then be analyzed using electron microscopy (EM) and immuno-EM methods. Subpopulations of COPI vesicles can be bound selectively using recombinant golgins. Alternatively, COPI vesicles can be attached to prebound Golgi membranes. Marking these vesicles selectively with biotin allows their site of attachment to be identified.

Original languageEnglish
Article number13
Pages (from-to)125-134
Number of pages10
JournalMethods in Enzymology
Volume404
DOIs
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Coat Protein Complex I
Assays
Electron microscopy
Membranes
Immunoelectron Microscopy
Biotin
Glass
Electron Microscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tethering assays for COPI vesicles mediated by golgins. / Satoh, Ayano; Malsam, Jörg; Warren, Graham.

In: Methods in Enzymology, Vol. 404, 13, 2006, p. 125-134.

Research output: Contribution to journalArticle

Satoh, Ayano ; Malsam, Jörg ; Warren, Graham. / Tethering assays for COPI vesicles mediated by golgins. In: Methods in Enzymology. 2006 ; Vol. 404. pp. 125-134.
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