Abstract
A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.
| Original language | English |
|---|---|
| Article number | 211114 |
| Journal | Applied Physics Letters |
| Volume | 96 |
| Issue number | 21 |
| DOIs | |
| Publication status | Published - May 24 2010 |
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ASJC Scopus subject areas
- Physics and Astronomy (miscellaneous)
Cite this
Terahertz chemical microscope for label-free detection of protein complex. / Kiwa, Toshihiko; Kondo, Yousuke; Minami, Yuji; Kawayama, Iwao; Tonouchi, Masayoshi; Tsukada, Keiji.
In: Applied Physics Letters, Vol. 96, No. 21, 211114, 24.05.2010.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Terahertz chemical microscope for label-free detection of protein complex
AU - Kiwa, Toshihiko
AU - Kondo, Yousuke
AU - Minami, Yuji
AU - Kawayama, Iwao
AU - Tonouchi, Masayoshi
AU - Tsukada, Keiji
PY - 2010/5/24
Y1 - 2010/5/24
N2 - A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.
AB - A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.
UR - http://www.scopus.com/inward/record.url?scp=77956247993&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77956247993&partnerID=8YFLogxK
U2 - 10.1063/1.3441408
DO - 10.1063/1.3441408
M3 - Article
AN - SCOPUS:77956247993
VL - 96
JO - Applied Physics Letters
JF - Applied Physics Letters
SN - 0003-6951
IS - 21
M1 - 211114
ER -