Terahertz chemical microscope for label-free detection of protein complex

Toshihiko Kiwa; Yousuke Kondo; Yuji Minami; Iwao Kawayama; Masayoshi Tonouchi; Keiji Tsukada

doi:10.1063/1.3441408

Terahertz chemical microscope for label-free detection of protein complex

Toshihiko Kiwa, Yousuke Kondo, Yuji Minami, Iwao Kawayama, Masayoshi Tonouchi, Keiji Tsukada

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO₂/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO₂ surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.

Original language	English
Article number	211114
Journal	Applied Physics Letters
Volume	96
Issue number	21
DOIs	https://doi.org/10.1063/1.3441408
Publication status	Published - May 24 2010

ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

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10.1063/1.3441408

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title = "Terahertz chemical microscope for label-free detection of protein complex",

abstract = "A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.",

author = "Toshihiko Kiwa and Yousuke Kondo and Yuji Minami and Iwao Kawayama and Masayoshi Tonouchi and Keiji Tsukada",

note = "Funding Information: This work was partially supported by the Industrial Technology Research Grant Program in 2006 from the New Energy and Industrial Technology Development Organization (NEDO) of Japan and a Grant-in-Aid for Young Scientists A (Grant no. 21686038).",

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doi = "10.1063/1.3441408",

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T1 - Terahertz chemical microscope for label-free detection of protein complex

AU - Kiwa, Toshihiko

AU - Kondo, Yousuke

AU - Minami, Yuji

AU - Kawayama, Iwao

AU - Tonouchi, Masayoshi

AU - Tsukada, Keiji

N1 - Funding Information: This work was partially supported by the Industrial Technology Research Grant Program in 2006 from the New Energy and Industrial Technology Development Organization (NEDO) of Japan and a Grant-in-Aid for Young Scientists A (Grant no. 21686038).

PY - 2010/5/24

Y1 - 2010/5/24

N2 - A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.

AB - A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.

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Terahertz chemical microscope for label-free detection of protein complex

Abstract

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