Terahertz chemical microscope for label-free detection of protein complex

Toshihiko Kiwa, Yousuke Kondo, Yuji Minami, Iwao Kawayama, Masayoshi Tonouchi, Keiji Tsukada

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO2/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO2 surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.

Original languageEnglish
Article number211114
JournalApplied Physics Letters
Volume96
Issue number21
DOIs
Publication statusPublished - May 24 2010

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biotin
microscopes
proteins
sapphire
immunoassay
pulses
sensitivity
lasers
interactions

ASJC Scopus subject areas

  • Physics and Astronomy (miscellaneous)

Cite this

Terahertz chemical microscope for label-free detection of protein complex. / Kiwa, Toshihiko; Kondo, Yousuke; Minami, Yuji; Kawayama, Iwao; Tonouchi, Masayoshi; Tsukada, Keiji.

In: Applied Physics Letters, Vol. 96, No. 21, 211114, 24.05.2010.

Research output: Contribution to journalArticle

Kiwa, Toshihiko ; Kondo, Yousuke ; Minami, Yuji ; Kawayama, Iwao ; Tonouchi, Masayoshi ; Tsukada, Keiji. / Terahertz chemical microscope for label-free detection of protein complex. In: Applied Physics Letters. 2010 ; Vol. 96, No. 21.
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