Terahertz chemical microscope for label-free detection of protein complex

Toshihiko Kiwa; Yousuke Kondo; Yuji Minami; Iwao Kawayama; Masayoshi Tonouchi; Keiji Tsukada

doi:10.1063/1.3441408

Terahertz chemical microscope for label-free detection of protein complex

Toshihiko Kiwa, Yousuke Kondo, Yuji Minami, Iwao Kawayama, Masayoshi Tonouchi, Keiji Tsukada

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22 Citations (Scopus)

Abstract

A terahertz (THz) chemical microscope (TCM) has been proposed and developed for label-free analysis of the interactions of proteins, including immune reactions. A SiO₂/Si/sapphire plate was fabricated and emitted THz pulses from the sapphire side using a femtosecond laser. The amplitude of a THz pulse depends on the chemical potential of the SiO₂ surface. As an initial demonstration, a biotin-avidin complex was analyzed using TCM. Results indicate that biotin concentrations of up to 10-12 mol/l could be detected, suggesting that a high sensitivity immunoassay system can be realized using this method. The distribution of the THz peak amplitude was measured, reflecting the distribution of the biotin-avidin complex on the plate.

Original language	English
Article number	211114
Journal	Applied Physics Letters
Volume	96
Issue number	21
DOIs	https://doi.org/10.1063/1.3441408
Publication status	Published - May 24 2010

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ASJC Scopus subject areas

Physics and Astronomy (miscellaneous)

Cite this

Kiwa, T., Kondo, Y., Minami, Y., Kawayama, I., Tonouchi, M., & Tsukada, K. (2010). Terahertz chemical microscope for label-free detection of protein complex. Applied Physics Letters, 96(21), [211114]. https://doi.org/10.1063/1.3441408

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AU - Tsukada, Keiji

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10.1063/1.3441408