Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation

Mika Ikegame, Yoshiaki Tabuchi, Yukihiro Furusawa, Mariko Kawai, Atsuhiko Hattori, Takashi Kondo, Toshio Yamamoto

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Mechanical stress promotes osteoblast proliferation and differentiation from mesenchymal stem cells (MSCs). Although numerous growth factors and cytokines are known to regulate this process, information regarding the differentiation of mechanically stimulated osteoblasts from MSCs in in vivo microenvironment is limited. To determine the significant factors involved in this process, we performed a global analysis of differentially expressed genes, in response to tensile stress, in the mouse cranial suture wherein osteoblasts differentiate from MSCs. We found that the gene expression levels of several components involved in bone morphogenetic protein, Wnt, and epithelial growth factor signalings were elevated with tensile stress. Moreover gene expression of some extracellular matrices (ECMs), such as cysteine rich protein 61 (Cyr61)/CCN1 and galectin- 9, were upregulated. These ECMs have the ability to modulate the activities of cytokines and are known as matricellular proteins. Cyr61/CCN1 expression was prominently increased in the fibroblastic cells and preosteoblasts in the suture. Thus, for the first time we demonstrated the mechanical stimulation of Cyr61/CCN1 expression in osteogenic cells in an ex vivo system. These results suggest the importance of matricellular proteins along with the cytokine-mediated signaling for the mechanical regulation of MSC proliferation and differentiation into osteoblastic cell lineage in vivo.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalBiomedical Research
Volume37
Issue number2
DOIs
Publication statusPublished - 2016

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Cranial Sutures
Cysteine-Rich Protein 61
Osteoblasts
Stem cells
Mesenchymal Stromal Cells
Tensile stress
Intercellular Signaling Peptides and Proteins
Cytokines
Gene expression
Extracellular Matrix
Proteins
Galectins
Gene Expression
Bone Morphogenetic Proteins
Mechanical Stress
Cell proliferation
Cell Lineage
Sutures
Cell Differentiation
Genes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation. / Ikegame, Mika; Tabuchi, Yoshiaki; Furusawa, Yukihiro; Kawai, Mariko; Hattori, Atsuhiko; Kondo, Takashi; Yamamoto, Toshio.

In: Biomedical Research, Vol. 37, No. 2, 2016, p. 117-126.

Research output: Contribution to journalArticle

Ikegame, Mika ; Tabuchi, Yoshiaki ; Furusawa, Yukihiro ; Kawai, Mariko ; Hattori, Atsuhiko ; Kondo, Takashi ; Yamamoto, Toshio. / Tensile stress stimulates the expression of osteogenic cytokines/growth factors and matricellular proteins in the mouse cranial suture at the site of osteoblast differentiation. In: Biomedical Research. 2016 ; Vol. 37, No. 2. pp. 117-126.
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